MagPure A3 Features:
- Technology Base:
- Utilizes Magen’s solid-phase paramagnetic bead technology for the purification of PCR amplicons.
- Compatibilidad de alto rendimiento:
- Designed for high-throughput applications, facilitating efficient processing of multiple samples.
AmPure Technology:
- Enlace selectivo:
- Features an optimized buffer that selectively binds PCR amplicons of 100bp and larger to paramagnetic beads.
- Contaminant Removal:
- Efficiently removes excess primers, nucleótidos, salts, and enzymes through a simple washing procedure.
- Purity of PCR Product:
- Delivers PCR products that are essentially free of contaminants, enhancing downstream application reliability.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure) |
| Aplicaciones | Secuenciación, gene chip and qPCR, etc.. |
| Tecnología de purificación | Tecnología de cuentas magnéticas |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | PCR products, enzymatic reaction solution |
| Cantidad de muestra | Appropriate |
| Volumen de elución | ≥10μl |
| Tiempo de operacion | ≤50 minutos |
Principio
Product Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- Contaminant Removal:
- Excess primers, nucleótidos, salts, and enzymes are removed via a simple washing procedure.
- DNA Elution:
- Purified DNA is eluted using Elution Buffer or Water.
Ventajas
- Wide application – DNA products, enzyme digestion system, PCR reaction
- Alta recuperación – suitable for 100bp-20kb fragments
- Alta pureza – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
- Strong applicability – manual operation or automatic workstation
Contenido del kit
| Contenido | BXP-5 | BXP-50 | BXP-500 |
| MagPure A3 | 5 ml | 50 ml | 500 ml |
Almacenamiento y estabilidad
Storage and Handling Instructions:
- Condiciones de almacenaje:
- Store MagPure A3 at 2-8°C upon arrival.
- Stable for up to 18 meses en estas condiciones.
- Short-term Storage:
- Short-term storage (hasta 12 semanas) a temperatura ambiente (15-25°C) does not affect performance.
- Reagent Preparation:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogenous and consistent.
NO CONGELAR.
Reseñas
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