This product provides fast and easy methods for the purification of total DNA for reliable PCR y transferencia del sur. ADN total (p.ej., genómico, plásmido) can be purified from bacterial cultures.
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation total DNA from bacterial culture |
| Aplicaciones | PCR, Southern blot y digestión enzimática, etc.. |
| Tecnología de purificación | Tecnología de cuentas magnéticas |
| método de proceso | Manual o automático |
| Tipo de ejemplo | Gram negative or positive bacteria |
| Cantidad de muestra | Bacterial culture: 0.5-1.5ml |
| Volumen de elución | ≥60μl |
| Tiempo por carrera | ≤60 minutos |
Principio
- This product utilizes high-binding magnetic particles for purification.
- Samples are lysed and digested using a lysate and Lysozyme.
- DNA is released into the lysate during this process.
- Magnetic particles and binding solution are added, allowing DNA to be adsorbed onto the surface of the particles while impurities such as proteins are not adsorbed.
- The adsorbed particles undergo washing with a washing solution to remove proteins and impurities.
- Ethanol is used for further washing to remove salts.
- Finally, DNA is eluted using Elution Buffer.
Ventajas
- Rápido: Multiple samples can be extracted within 60 minutos (después de la digestión), making the process time-efficient.
- Alta pureza: Unique adsorbents ensure the complete removal of inhibitory factors, resulting in high-purity DNA extraction.
- Alta recuperación: DNA can be recovered at the level of picograms (PG), indicating a high level of efficiency in the extraction process.
Contenido del kit
| Contenido | D513101 | D513102 | D513103 |
| Tiempos de purificación | 48 preparativos | 96 preparativos | 480 preparativos |
| Partículas MagPure | 1.2 ml | 2.5 ml | 11 ml |
| lisozima | 60 mg | 120 mg | 600 mg |
| Proteinasa K | 24 mg | 50 mg | 220 mg |
| Tampón de disolución de proteasa | 3 ml | 6 ml | 30 ml |
| Buffer Ficha de datos de seguridad (MSDS) | 1.5 ml | 3 ml | 15 ml |
| MLA de búfer | 30 ml | 60 ml | 300 ml |
| Tampón GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Almacenamiento y estabilidad
- Partículas MagPure, lisozima, and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage (hasta 12 semanas) a temperatura ambiente (15-25°C) no afecta su rendimiento.
- Los componentes restantes del kit se pueden almacenar secos a temperatura ambiente. (15-25°C) and remain stable for at least 18 meses en estas condiciones.
Reseñas
Aún no hay reseñas.