Introducción
This kit empowers researchers with a streamlined solution for isolating high-quality circulating cell-free DNA (cfDNA) from various biofluids:
- Sample Types: Plasma, Suero (1-6ml)
- Objetivo: cfDNA molecules
- Downstream Applications:
- PCR (polymerase chain reaction)
- PCR en tiempo real
- Biochip analysis
- Next-generation sequencing (NGS)
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation circulating DNA from 1-6ml plasma, suero, fluidos corporales |
| Aplicaciones | qPCR, NGS, etc.. |
| Tecnología de purificación | Tecnología de cuentas magnéticas |
| método de proceso | Manual o automático |
| Tipo de ejemplo | Suero, plasma |
| Cantidad de muestra | 1-6ml |
| Volumen de elución | ≥50μl |
| Tiempo por carrera | ≤60 minutos |
Principio
This product utilizes innovative magnetic bead technology for efficient DNA extraction:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
- Binding to Magnetic Beads: Magnetic particles coated for high DNA affinity are added to the lysate. DNA molecules selectively bind to the surface of these beads, while impurities like proteins remain unbound.
- Washing and Purification: A series of washes with different solutions remove unbound proteins and other cellular debris.
- Elution: A special elution buffer disrupts the interaction between the DNA and the magnetic beads, allowing the purified DNA to be collected in a separate tube.
Ventajas
- Economía – menos que 50% del precio de Qiagen y otros productos importados
- Automatic – without labor
- Alta pureza – DE 260 / 280: 1.7-1.9, DE 260 / 230:1.5-2.0
Contenido del kit
| Contenido | IVD5435 |
| Tiempos de purificación | 50 |
| Partículas MagPure F | 14 ml |
| Carrier RNA | 310 µg |
| Proteinasa K | 240 mg |
| Tampón de disolución de proteasa | 15 ml |
| Buffer Ficha de datos de seguridad (MSDS) | 15 ml |
| Buffer MLK | 500 ml |
| Buffer MAW1 | 250 ml |
| Tampón MW2* | 50 ml |
| Tampón de elución | 60 ml |
Almacenamiento y estabilidad
Ideal Storage:
- Store Proteinase K, Carrier RNA, and MagPure Particles F in the refrigerator (2-8°C) upon arrival for maximum effectiveness.
Acceptable Short-Term Storage:
- These components can be kept at room temperature (15-25°C) for up to 12 weeks without impacting their performance.
Storing Remaining Components:
- All other kit components can be stored dry at room temperature (15-25°C).
- They will remain stable for at least 18 meses en estas condiciones.
Important Notes:
- If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
- Always ensure all buffers reach room temperature before using them for optimal results.
Datos del experimento
Reseñas
Aún no hay reseñas.