MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidina isotiocianato, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, planta, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, escuela politécnica(A) + selection, traducción in vitro, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | RNA isolation solvent (substitution for Trizol/Qiazol reagent) |
| Aplicaciones | RT-PCR, hibridación del norte, escuela politécnica (a) enrichment, etc.. |
| Tecnología de purificación | Acid phenol guanidine isothiocyanate |
| método de proceso | Manual (centrifugation) |
| Tipo de ejemplo | Tejido animal, plant tissue, adherent cells, suspended cells, bacteria, trace fungi, etc.. |
| Cantidad de muestra | Flexible |
| Volumen de elución | Variation with sample size |
| Tiempo por carrera | Variation with sample size |
Ventajas
- Extensive – suitable for various kinds of biological samples, including animals, plants, células cultivadas, bacteria, etc..
- Alto rendimiento – efficient cleavage releases more RNA
- Alta pureza – purified RNA is suitable for various downstream applications
- Flexible – sample amount can be adjusted according to the demand
- High cost performance compared with similar products
Almacenamiento y estabilidad
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 meses bajo la condición. Sin embargo, almacenamiento a corto plazo (hasta 12 semanas) a temperatura ambiente (15-25°C) no afecta su rendimiento.
Datos del experimento
Reseñas
Aún no hay reseñas.