Instructions:
- Sample Collection:
- Collect 0.1-1ml blood sample.
- Preparación de la muestra:
- Agregar 2 a 3 times the volume of 1x red blood cell solution to the blood sample.
- Mix thoroughly by inverting.
- Centrifugar en 12,000 rpm para 1 minuto.
- Carefully aspirate the supernatant. The precipitate should ideally be white or light red.
- Agregar 400 μl reagent buffer I and 10 μl epoxy resin K (10 mg/ml) to the precipitate.
- Vortex and mix for 15 segundos, then let it sit for 2 minutos.
- Special Case: Low-level Organisms:
- If processing blood from low-level organisms (p.ej., aves de corral, birds) containing cell nuclei:
- Add 1x red blood cell solution and directly add 200 μl of reagent buffer I (total volume not exceeding 500 µl).
- Let it sit for 2 minutos.
- Digestion:
- Agregar 10 μl epoxy resin K (10 mg/ml) to the mixture.
- Invert and mix, then digest at 65°C for 10 minutos.
- Durante este periodo, invertir y mezclar 6-7 times to ensure complete digestion.
- Transfer to Reagent Plate:
- Add the solution from the previous step to the 96-well reagent plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.
