Pre-processing of DNA extraction from animal tissue 48 Samples

Preprocesamiento de la extracción de ADN de tejido animal. 48 Muestras

Name: Pre-processing of DNA extraction from animal tissue 48 Samples
SKU: 196
Price: 225.00 USD
Availability: InStock

$225.00

Envío USD 45 - Gratis sobre USD 300

DTE es una plataforma de comercio electrónico con sede en China que se especializa en ventas en línea de pruebas moleculares., ELISA, y productos relacionados.

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Descripción

Sample Collection:
- Recolectar 0.1-1 ml blood sample.
Preparación de la muestra:
- For blood samples, agregar 2-3 times the volume of 1x erythrocyte lysate.
- Fully invert and mix well.
- Centrifugar en 12,000 rpm para 1 minuto.
- Carefully remove the supernatant. The precipitate should be white or light red.
- Agregar 400 μl reagent buffer I and 10 μl proteinase K (10 mg/ml) to the precipitate.
- Vortex and mix for 15 segundos, then let it sit at room temperature for 2 minutos.
Special Case: Low-level Organisms:
- If processing blood from poultry, birds, or other low-level organisms where red blood cells contain nuclei:
- Skip adding 1x red blood cell lysate.
- Directly add 200 μl of reagent buffer I (total volume not exceeding 500 µl).
- Let it sit at room temperature for 2 minutos.
Digestion:
- Agregar 10 μl proteinase K (10 mg/ml) to the mixture.
- Thoroughly mix by inversion.
- Digest at 65°C for 10 minutos.
- Invert and mix 6-7 times during digestion until complete.
Transfer to Reagent Plate:
- Add the lysate from the previous step to the 96-well reagent plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.