Name: Pre-processing of plant sample genomic DNA extraction 96 Samples
SKU: 200
Price: 225.00 USD
Availability: InStock

Pre-processing of plant sample genomic DNA extraction 96 Muestras

$225.00

Envío USD 45 - Gratis sobre USD 300

DTE es una plataforma de comercio electrónico con sede en China que se especializa en ventas en línea de pruebas moleculares., ELISA, y productos relacionados.

Fabricante: Marcas chinas líderes
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Manejo de muestras:
- A. Material Collection & Almacenamiento:
  - If collected fresh materials cannot be used immediately, cool them in liquid nitrogen and store them at -80°C. Dried materials can be stored in the refrigerator.
- B. Collection Preference:
  - Collect fresh material whenever possible, as it contains fewer polysaccharides and polyphenols.
- C. Fungal Sample Collection:
  - Fungi can be collected by centrifugation from liquid media.
Preparación de la muestra:
- Grind about 100 mg de muestras frescas o no más de 20 mg of dry matter with liquid nitrogen.
- Agregar 400 μl Buffer I and 10 μl RNaseA (10 mg/ml) to ensure no lumpy tissue in the sample, which can reduce DNA yield.
- Incubar a 65°C durante 2-10 minutos, invert, y mezclar 2-3 times during this period. Extend incubation time if the sample is difficult to lyse, but not more than 1 hora.
- Agregar 130 μl Buffer II, mezclar bien, and incubate on ice for 5 minutes to precipitate polysaccharides and proteins.
Purification:
- Add the lysate to the purification column and centrifuge at 13,000 rpm para 2 minutos.
Transfer to Reagent Plate:
- Add the centrifuged supernatant from the previous step to the 96-well plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.