Solarbio Anti-A and Anti-B Blood Grouping Reagents (Monoclonal Antibody)

The main components of anti-A and anti-B blood typing reagent (monoclonal antibody)/anti-A and anti-B serum are hybridoma cell culture supernatant which secretes anti-human type A and anti-human type B blood type antigen monoclonal antibody.

Especificación

Principle of the Test:

• This product is prepared from a hybridoma cell culture supernatant producing anti-A or anti-B blood group antibodies (or with a small amount of homologous ascites added to the supernatant).
• It uses the principle of agglutination test.
• Specifically designed for identifying human ABO blood type.

Main Components:

1. Anti-A blood typing reagent (blue) 10ml, 1 botella
2. Anti-B blood typing reagent (yellow) 10ml, 1 botella
3. Instruction manual, 1 sheet

Almacenamiento:

• Store at 2-8℃, evitar la luz, validity period of 12 meses

Requisitos de muestra:

• This reagent is suitable for whole blood or 10%, 5% red blood cell suspension.

Test Methods:

1. Slide Method:
   • Mix this product with the subject’s whole blood or 10% red blood cell physiological saline suspension at a ratio of 1:1, with no further dilution needed, and determine the result based on the presence or absence of agglutination.
2. Test Tube Method:
   • Mix this product with the subject’s 5% red blood cell physiological saline suspension at a ratio of 1:1, no further dilution needed, mezclar bien, centrifuge at 1000rpm for 1 minute or let stand at room temperature for 1 hora, and determine the result based on the presence or absence of agglutination.

Interpretación de resultados

Nota: ‘+’ indicates agglutination reaction, and ‘-‘ indicates no agglutination reaction.

The interpretation of the test results:

• If agglutination occurs when mixing known anti-A blood typing reagent with the subject’s red blood cells, it indicates blood type A.
• If agglutination occurs when mixing known anti-B blood typing reagent with the subject’s red blood cells, it indicates blood type B.
• If agglutination occurs when mixing both known anti-A and anti-B blood typing reagents with the subject’s red blood cells, it indicates blood type AB.
• If no agglutination occurs when mixing both known anti-A and anti-B blood typing reagents with the subject’s red blood cells, it indicates blood type O.

Limitations of the test method:

• This test is only used for positive determination of ABO blood group.

Product performance index:

• [Affinity] < 15 segundos, the titer shall not be lower than the national reference product synchronous determination results.

Anti-A and anti-B Blood typing reagent notes:

1. For subjects with more self-cold agglutination, blood type identification is often mistaken for AB blood type. In this case, it is necessary to:
   • Wash the subject’s cells with physiological sodium chloride solution at 37℃ 2-3 times to remove the lectin adsorbed on the red blood cells, and then identify the blood type.
2. When the matching test is done:
   • If there is a mismatch, take the serum of the subject and perform reverse stereotyping tests with known A blood group or B blood group cells to verify that the originally identified blood type is correct.
3. Subtypes (such as Ax) cannot be detected by the immediate test tube method, and the action time needs to be extended.
4. This product should not be used if there is turbidity or discoloration. If the bottle is opened and used for a long time:
   • Check with known ABO blood group red blood cells to ensure the result is consistent with the known blood type, preventing product contamination, deterioration, and failure, which could lead to identification errors.
5. Use within the validity period.