- Cuantitativo PCR (qPCR) Overview:
- qPCR, also known as Real-time PCR, is a widely used technology for precise analysis of gene expression.
- It can be categorized into dye-based and probe-based methods, with dye-based being more popular, convenient, y rentable.
- Dye-based qPCR monitors real-time fluorescence of the dye binding to double-stranded DNA to measure DNA amplification indirectly during each cycle.
- Ct value (Cq value) is determined when the fluorescence signal is distinctly detected over the background, which can be used to evaluate target abundance or calculate absolute quantities.
- SYBR Green 2xqPCR Mix Master Mix:
- Provides superior specificity, robust amplification efficiency, ideal reproducibility, and stability in quantifying target DNA or cDNA.
- It’s a 2X premix containing hot-start Taq polymerase combined with an antibody for preferable specificity and high amplification speed.
- SYBR Green I in the mix emits fluorescence when bound to double-stranded DNA amplified in each cycle, allowing for indirect quantification of amplification products in real-time.
- Use SYBR or SYBR/FAM scan mode setting on the real-time instrument.
- Limitations of Dye-based qPCR:
- SYBR Green I can intercalate into any double-stranded DNA, including primer-dimers or other undesired products, causing nonspecific fluorescence.
- To confirm specificity, perform a melt curve analysis after amplification, where a sharp peak around the annealing temperature is ideal.
Mezcla SYBR Green 2xqPCR 1ml *10
$80.00
Envío USD 45 - Gratis sobre USD 300DTE es una plataforma de comercio electrónico con sede en China que se especializa en ventas en línea de pruebas moleculares., ELISA, y productos relacionados.
- Fabricante: Marcas chinas líderes
- Envío: Envío acelerado de FedEx directamente desde las fábricas
- Elegible para devolución o reemplazo dentro de 30 días
- Métodos de pago: PayPal seguro o tarjeta de crédito.
Descripción
Información adicional
| Peso | 0.7 kg |
|---|---|
| nombre de la marca |
Reseñas (0)
Adjunto
Productos relacionados
- Reactivo de PCR en tiempo real
ADN polimerasa de arranque en caliente Sanshibio Taq
$38.00 – $274.00 - Reactivo de PCR en tiempo real
Kit de detección de mutaciones del gen EGFR humano (Método de PCR-fluorescencia) 12T para la investigación
$1,300.00
Reseñas
Aún no hay reseñas.