Introduction
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-RAP, Northern hybridization, etc.. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc..
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA from bacteria culture |
| Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Bacteria culture |
| Montant de l'échantillon | Bactéries: <10^9 |
| Volume d'élution | ≥30μl |
| Temps par course | ≤40 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (tel que guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Avantages
- Rapide – several samples can be extracted in 30 minutes
- Haute pureté – the purified RNA can be directly used in various downstream applications
- Récupération élevée – RNA can be recovered at the level of PG
- Bonne répétabilité – silica gel column purification technology can obtain ideal results every time
- Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
- Sufficient components – the kit contains carried lysozyme and glass beads
Contenu du kit
| Contenu | R418102 | R418103 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Mini Columns | 50 | 250 |
| 2Tubes de prélèvement ml | 50 | 250 |
| Glass Beads (0.1-0.2mm) | 20 g | 90 g |
| Lysozyme | 90 mg | 400 mg |
| Tampon de dissolution de protéase | 5 ml | 15 ml |
| Buffer STE | 10 ml | 30 ml |
| MagZol Reagent | 60 ml | 270 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Stockage et stabilité
MagZol Reagent and Lysozyme should be stored at 2-8°C upon arrival. Les composants restants du kit peuvent être stockés au sec à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. L'ensemble du kit peut être conservé entre 2 et 8°C, mais dans ce cas, les tampons doivent être redissous avant utilisation. Assurez-vous que tous les tampons sont à température ambiante lorsqu'ils sont utilisés.
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