Introduction
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement de l'ARN total et du miARN des cellules et des tissus sans réactif MagZol |
| Applications | RT-RAP, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Cellule, Animal tissue, Plant tissue |
| Montant de l'échantillon | Cells: ≤ 5 x 10^6, Animal tissue:<10mg |
| Volume d'élution | ≥30μl |
| Temps par course | ≤40 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, y compris les miARN, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.
Avantages
- High quality – one step Extraction d'ARN reagent combined with silica gel column can obtain the highest concentration
- Rapide – several samples can be extracted in 40 minutes
- High applicability – samples including animals, plantes, bactéries, cellules, etc..
- Haute concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
- Safe – no phenol chloroform extraction and no use of Trizol reagent
Contenu du kit
| Contenu | R431102 | R431103 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Mini Columns | 100 | 2 X 250 |
| 2Tubes de prélèvement ml | 100 | 2 X 250 |
| Protéinase K | 48 mg | 240 mg |
| Tampon de dissolution de protéase | 5 ml | 15 ml |
| Buffer RLC | 40 ml | 200 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 60 ml |
Stockage et stabilité
Proteinase K should be stored at 2-8°C upon arrival. Cependant, stockage à court terme (jusqu'à 12 semaines) à température ambiante (15-25°C) n'affecte pas leurs performances. Les composants restants du kit peuvent être conservés à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions.
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