Introduction
- Target: Short DNA fragments (<1000pb) in the blood (tumor markers, fetal DNA) ou de l'urine.
- Efficiency: Purifies circulating nucleic acids from human biofluids (plasma, sérum, urine).
- Applications: Extracted DNA is suitable for approved clinical diagnostic tests.
Benefits:
- Optimized for short DNA analysis.
- Works with various human fluids.
- A larger format processes more samples (potentially higher DNA yield).
- Directly usable in clinical tests.
This Midi Kit empowers researchers and clinical labs for applications in oncology, NIPT, and liquid biopsies.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement de l'ADN circulant à partir de 1 à 5 ml de plasma, sérum, and body fluids using vacuum protocol |
| Applications | qPCR, analyse de copeaux liquides ou solides, hybridization SNP detection, etc.. |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (vide) |
| Échantillon type | Sérum, plasma, and other cell-free fluid samples |
| Montant de l'échantillon | 1-5ml |
| Volume d'élution | ≥50μl |
| Temps par course | ≤60 minutes |
| Volume de liquide transporté par colonne | 4ml |
| Rendement de liaison de la colonne | 1mg |
Principe
This product offers a streamlined method for isolating DNA using silica column technology:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
- Binding to Silica: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.
- Washing: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.
- Elution: A low-salt buffer is used to elute (release) the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.
Key Advantages:
- Efficient Purification: Silica column technology offers a fast and reliable process for isolating DNA from complex biological samples.
- High-Purity DNA: The selective binding properties of the silica membrane ensure the purification of high-quality DNA, suitable for various downstream applications (RAP, etc.).
- Simplified Workflow: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Avantages
- Haut rendement – processus le plus optimal, ADN libre (>50pb) peut être obtenu dans la mesure maximale
- Haute concentration – faible volume d'élution, assurer une concentration élevée d’acide nucléique
- Haute pureté – méthode à faible liaison alcoolique, éliminant complètement la pollution par les inhibiteurs et les protéines
- Récupération élevée – DNA can be recovered at the level of PG by silica gel column purification
Contenu du kit
| Contenu | DIV3182 |
| Temps de purification | 50 |
| Liste de contrôle d'accès tampon | 250 ml |
| Tampon ACB* | 300 ml |
| Tampon DCW1* | 22 ml |
| Tampon DCW2* | 10 ml |
| Protéinase K | 540 mg |
| Tampon de dissolution de protéase | 30 ml |
| ARN porteur | 110 µg |
| Eau sans nucléase | 20 ml |
| Mini-colonnes HiPure CFDNA | 50 |
| 2 Tubes de prélèvement ml | 100 |
| Tube d'extension | 50 |
| Connecteur Vac | 50 |
Stockage et stabilité
- Protéinase K & RNA Carrier: Fridge (2-8°C) for best results.
- Other Kit Components: Température ambiante (15-25°C) is good.
- Storing Cold? Warm buffers up before use.
Données d'expérimentation
Commentaires
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