Introduction
HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:
- Targets:
- Tumor-derived cfDNA fragments and mRNAs in blood
- Fetal nucleic acids in maternal blood
- Sample Types:
- Human plasma
- Human serum
- Human urine (fresh or frozen once)
- Focus on Short DNA: Captures short nucleic acids (<1000pb) commonly found in biofluids.
- Efficient Purification: Effectively recovers cfDNA from various biofluids.
- Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.
Benefits:
- Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
- Broad Sample Compatibility: Works with various human biofluids, offering flexibility in sample selection.
- Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
- Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.
This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsies, ultimately aiding in patient diagnosis and management.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement de l'ADN circulant à partir de 1 à 5 ml de plasma, sérum, body fluids by spin |
| Applications | qPCR, analyse de copeaux liquides ou solides, hybridization, and SNP detection, etc.. |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation) |
| Échantillon type | Sérum, plasma, and other cell-free fluid samples |
| Montant de l'échantillon | 1-5ml |
| Volume d'élution | ≥50μl |
| Temps par course | ≤70 minutes |
| Volume de liquide transporté par colonne | 4ml |
| Rendement de liaison de la colonne | 1mg |
Principe
This product utilizes silica gel columns for efficient DNA extraction:
- Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
- Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
- Washing: Wash buffers to remove leftover proteins and cellular debris.
- Elution: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Avantages
- Haut rendement – processus le plus optimal, ADN libre (>50pb) peut être obtenu dans la mesure maximale
- Haute concentration – faible volume d'élution, assurer une concentration élevée d’acide nucléique
- Haute pureté – méthode à faible liaison alcoolique, éliminant complètement la pollution par les inhibiteurs et les protéines
- Récupération élevée – DNA can be recovered at the level of PG by silica gel column purification
Contenu du kit
| Contenu | D318203D |
| Temps de purification | 50 Préparations |
| Liste de contrôle d'accès tampon | 250 ml |
| Tampon ACB* | 300 ml |
| Tampon DCW1* | 22 ml |
| Tampon DCW2* | 10 ml |
| Protéinase K | 540 mg |
| Tampon de dissolution de protéase | 30 ml |
| ARN porteur | 110 µg |
| Eau sans nucléase | 20 ml |
| Mini-colonnes HiPure CFDNA | 50 |
| 2 Tubes de prélèvement ml | 100 |
| Extender Tubes | 50 |
| Sealing O-Ringes | 50 |
| 50ml Collection Tube | 50 |
| Support Tube | 50 |
Stockage et stabilité
- Protéinase K & ARN porteur: Fridge (2-8°C) for best results.
- Everything Else: Température ambiante (15-25°C) is fine.
- Important: If storing cold, warm buffers before use.
Commentaires
Il n'y a pas encore de critiques.