HiPure DNA Clean Up 96 Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, RAP, séquençage, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Recover DNA fragments between 60bp-10kbp from PCR, Reactions, crude DNA using 96 well Bind Plate |
| Applications | RAP, séquençage, labeling reactions, ligations and restriction digestion, etc.. |
| Méthode de purification | 96 well Bind Plate |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | PCR product, enzymatic reaction |
| Montant de l'échantillon | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>Appropriate |
| Recovery | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥80% |
| Volume d'élution | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥75μl |
| Temps par course | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤60 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 20µg |
Principe
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Avantages
- High recovery efficiency – ≥80% DNA recovery
- Rapide – isolation can be completed in 60 minutes
Contenu du kit
| Contenu | D212201 | D212202 | D212203 |
| Temps de purification | 1 X 96 Préparations | 4 X 96 Préparations | 20 X 96 Préparations |
| Buffer GDP | 120 ml | 400 ml | 4 X 400 ml |
| Buffer DW2 | 20 ml | 100 ml | 3 X 100 ml |
| Tampon d'élution | 20 ml | 20 ml | 30 ml |
| HiPure DNA Plate | 1 | 4 | 20 |
| 2.2ml Collection Plate | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.8 ml Collection Plate | 1 | 4 | 20 |
Stockage et stabilité
The Kit can be stored dry at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
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