The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, cellule, tissus, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-RAP, Transfert du Nord, poly A+ RNA(ARNm) purification, nuclease protection and in vitro translation.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent |
| Applications | RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice, DNA filtration technology |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Simple plant, petal, peel, seed |
| Montant de l'échantillon | 50-150mg |
| Volume d'élution | ≥300μl |
| Temps par course | ≤15 minutes（1-24 samples） |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
Avantages
- Efficient DNA removal – one step Extraction d'ARN can effectively remove genomic DNA
- High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Rapide – several samples can be extracted in 15 minutes by column method
- Wide range – samples including animals, plantes, bactéries, cellules, etc.
- High output – large sample amount, and the yield can be up to 200μg
Contenu du kit
| Contenu | R401402 | D401403 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Mini Columns | 50 | 250 |
| 2Tubes de prélèvement ml | 50 | 250 |
| PlantZol Reagent | 60 ml | 200 ml |
| Buffer GXP | 30 ml | 150 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Stockage et stabilité
The Kit can be stored dry at room temperature (15–25°C) et sont stables pendant au moins 18 mois dans ces conditions. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
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