This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (génomique, mitochondriale, and chloroplast) for reliable RAP and southern blot in less than 1 heure.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total DNA from 3g plant and fungal tissue |
| Applications | RAP, SSR, AFLP, RAPD and southern blot, etc.. |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Various plant samples (including conventional, polysaccharides and polyphenols) |
| Montant de l'échantillon | Fresh / frozen plant samples: 2-3 g Dried plant / seed samples: 0.5-1 g |
| Volume d'élution | ≥500μl |
| Temps par course | ≤120 minutes |
| Volume de liquide transporté par colonne | 20ml |
| Rendement de liaison de la colonne | 5mg |
Principe
Ce produit est basé sur la purification sur colonne de silice. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Avantages
- Broad spectrum – suitable for extracting DNA from various plant samples
- Rapide – several samples can be extracted in 30 minutes by silica technology
- Haute pureté – high quality DNA, completely remove inhibitors
- Haut rendement – silica technology can achieve the highest yield
Contenu du kit
| Contenu | D316302 | D316303 |
| Temps de purification | 10 Préparations | 50 Préparations |
| RNase A | 20 mg | 90 mg |
| Tampon de dissolution de protéase | 1.8 ml | 10 ml |
| Buffer PAL | 180 ml | 900 ml |
| Buffer GWP | 150 ml | 800 ml |
| Tampon GW2 | 25 ml | 200 ml |
| Tampon AE | 30 ml | 120 ml |
| HiPure DNA Maxi Columns II | 10 | 50 |
| 50 Tubes de prélèvement ml | 20 | 100 |
Stockage et stabilité
RNase A should be stored at 2-8°C upon arrival. Cependant, stockage à court terme (jusqu'à 12 semaines) à température ambiante (15-25°C) does not affect its performance. Les composants restants du kit peuvent être stockés au sec à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. L'ensemble du kit peut être conservé entre 2 et 8°C, mais dans ce cas, les tampons doivent être redissous avant utilisation. Assurez-vous que tous les tampons sont à température ambiante lorsqu'ils sont utilisés.
Guide d'achat
| Nom | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Volume d'élution | Yield (muscle) |
| HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100µl | 3-70µg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250µg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100µl | 3-50µg |
| HiPure SF Plant DNA 96 Trousse | D3167 | 50mg | 15mg | 96 well plate | 75 – 150µl | 3-30µg |
Commentaires
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