Introduction
The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica-membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation of total RNA from 0.25ml body fluids using column and MagZol LS reagent |
| Applications | qPCR / RT-RAP, liquid or solid-phase chip analysis, hybridization, and SNP detection |
| Méthode de purification | Mini colonne d'essorage |
| Technologie de purification | Technologie de la silice, acid phenol / guanidine extraction technology (MagZol pretreatment technology) |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Sang, sérum, plasma |
| Montant de l'échantillon | 250µl |
| Volume d'élution | ≥20µl |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (jusqu'à 100 µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.
Avantages
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Fast – isolation of several samples can be completed in 40 minutes by using the column purification method
- Sécurité – no phenol-chloroform extraction is required
- Sensitive – direct lysis of blood, plasma, and other samples without separation of leukocytes
Contenu du kit
| Contenu | R416302 | R416303 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Mini Columns | 50 | 250 |
| 2Tubes de prélèvement ml | 50 | 2 X 125 |
| MagZol LS Reagent | 60 ml | 270 ml |
| Buffer RWC | 20 ml | 60 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Stockage et stabilité
MagZol LS Reagent should be stored at 2-8°C upon arrival. Cependant, stockage à court terme (jusqu'à 24 semaines) à température ambiante (15-25°C) n'affecte pas leurs performances. Les composants restants du kit peuvent être stockés au sec à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions.
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