The Kit provides fast purification of high-quality RNA from plants, cellule, tissus, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-RAP, Transfert du Nord, poly A+ RNA (ARNm) purification, nuclease protection, and in vitro translation.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA from <150 mg simple plant sample (including plant sample containing starch) without chloroform |
| Applications | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice, DNA filtration technology |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Economic crops |
| Montant de l'échantillon | ≤150 mg |
| Volume d'élution | ≥30μl |
| Temps par course | ≤25 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanolis added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Avantages
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Rapide – several samples can be extracted in 25 minutes by column method
- Safe – no phenol chloroform extraction required
- Sensitive – RNA can be recovered at the level of PG
- Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Contenu du kit
| Contenu | R415102 | D415103 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure DNA Mini Column II | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2Tubes de prélèvement ml | 100 | 2 X 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer PRC1 | 50 ml | 200 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Stockage et stabilité
The Kit can be stored dry at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. Assurez-vous que tous les tampons sont à température ambiante lorsqu'ils sont utilisés. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Commentaires
Il n'y a pas encore de critiques.