Introduction

• HiPure ADN plasmidique Plus 96 Trousse:
  • Enables up to 96 mini-preps to be performed simultaneously.
  • Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96).
  • Provides a fast, simple, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications.
• Key Features:
  • Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries.
  • Plasmid DNA purified with the kit is immediately ready for use.
  • Eliminates the need for phenol extraction and ethanol precipitation.
  • High-quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Détails

Caractéristiques

Principe

• HiPure Plasmid Procedure:
  • Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
  • Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps.
• Procedure Steps:
  1. Preparation and Clearing of Bacterial Lysate:
     • The alkaline method is used to prepare and clear bacterial lysate.
  2. DNA Binding:
     • Supernatant transferred to a column to bind DNA to the silica membrane.
  3. Washing and Elution:
     • Proteins and other impurities washed away.
     • L'acide nucléique a finalement été élué avec un tampon à faible teneur en sel (10mmTris, pH9,0, 0.5mmEDTA).

Avantages

• Haute pureté – purified plasmid can be directly used in sequencing, enzyme digestion PCR, etc..
• Rapide – it takes only 60 minutes to complete the isolation
• Haut rendement – up to 1mg plasmid can be bound in one column
• Économie – high cost-performance

Contenu du kit

Stockage et stabilité

The kit components can be stored dry at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After the addition of RNase A， Buffer P1 is stable for 6 months when stored at 2-8°C.

Guide d'achat