Introduction
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-RAP, Transfert du Nord, poly A+ RNA (ARNm) purification, nuclease protection, and in vitro translation.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA and miRNA from 0.2-0.6ml serum and plasma |
| Applications | |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | |
| Montant de l'échantillon | |
| Yield | |
| Volume d'élution | |
| Temps par course | |
| Volume de liquide transporté par colonne | |
| Rendement de liaison de la colonne |
Principe
HiPure RNA technology simplifies total RNA isolation. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After the removal of protein the binding conditions for nucleic acids are adjusted by adding isopropanol. Total nucleic acids are bound to the column. Optionally, DNA can be removed by an on-column DNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-Free water.
Contenu du kit
| Contenu | R431402 | R431403 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Mini Columns | 100 | 2 X 250 |
| 2Tubes de prélèvement ml | 100 | 2 X 250 |
| Buffer CFL | 6 ml | 30 ml |
| Buffer CFP | 1.8 ml | 10 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 60 ml |
Stockage et stabilité
The kit components can be stored at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions.
Commentaires
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