This product provides a fast and easy way to purify DNA from plant and fungal tissue. Jusqu'à 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (génomique,mitochondriale, and chloroplast) for reliable RAP and southern blotting in less than 1 heure. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total DNA from100 mg simple plant |
| Applications | RAP, SSR, AFLP, RAPD and southern blot, etc.. |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Economic plant samples |
| Montant de l'échantillon | Fresh / frozen plant samples: ≤100mg gDried plant / seed samples: ≤20mg |
| Volume d'élution | ≥30μl |
| Temps par course | 30-50 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Avantages
- Safe – require no phenol or chloroform extraction
- Rapide – several samples can be extracted in 30 minutes by silica technology
- Haute pureté – high quality DNA, completely remove inhibitors
- Haut rendement – silica technology can achieve the highest yield
Contenu du kit
| Contenu | D316402 | D316403 |
| Temps de purification | 50 Préparations | 250 Préparations |
| RNase A | 10 mg | 50 mg |
| Tampon de dissolution de protéase | 1.8 ml | 5 ml |
| Buffer SPL | 30 ml | 150 ml |
| Buffer PS | 10 ml | 50 ml |
| Tampon GW1 | 22 ml | 110 ml |
| Tampon GW2* | 12 ml | 2 X 50 ml |
| Tampon AE | 15 ml | 60 ml |
| HiPure gDNA Mini Columns | 50 | 2 X 125 |
| 2 Tubes de prélèvement ml | 50 | 2 X 125 |
Stockage et stabilité
RNase A should be stored at 2-8°C upon arrival. Cependant, stockage à court terme (jusqu'à 24 semaines) à température ambiante (15-25°C) does not affect its performance. Les composants restants du kit peuvent être stockés au sec à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions.
Données d'expérimentation
Guide d'achat
| Nom | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Volume d'élution | Yield (muscle) |
| HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100µl | 3-70µg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250µg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100µl | 3-50µg |
| HiPure SF Plant DNA 96 Trousse | D3167 | 50mg | 15mg | 96 well plate | 75 – 150µl | 3-30µg |
Commentaires
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