Introduction
HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-RAP, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA from 500 mg soil sample |
| Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Forest soil, grassland soil, mining area soil, sediment and other samples |
| Montant de l'échantillon | 500 mg |
| Volume d'élution | ≥30μl |
| Temps par course | ≤60 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
The hipure silica gel column is based on a glass fiber filter membrane with a high binding force. Under the condition of a high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonds and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Avantages
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Rapide – extraction of several samples can be completed in 60 minutes by the column purification method
- Safe – no phenol-chloroform extraction required
- Sensitive – RNA can be purified at the level of PG
Contenu du kit
| Contenu | R418302 | R418303 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Micro Columns | 50 | 250 |
| 2Tubes de prélèvement ml | 100 | 500 |
| gDNA Filter Column | 50 | 250 |
| 2ml Beads Tubes | 50 | 250 |
| Buffer SOL | 30 ml | 150 ml |
| Tampon FDS | 4 ml | 15 ml |
| Buffer PHC | 30 ml | 150 ml |
| Buffer GDP | 40 ml | 150 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2 * | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Stockage et stabilité
Buffer PHC should be stored at 2–8°C upon arrival. Cependant, stockage à court terme (jusqu'à 12 semaines) à température ambiante (15–25°C) n'affecte pas leurs performances. Les composants restants du kit peuvent être stockés au sec à température ambiante (15–25°C) et sont stables pendant au moins 18 mois dans ces conditions. L'ensemble du kit peut être conservé entre 2 et 8°C, mais dans ce cas, les tampons doivent être redissous avant utilisation. Assurez-vous que tous les tampons sont à température ambiante lorsqu'ils sont utilisés.
Données d'expérimentation
Commentaires
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