Présentation du produit
- Offers fast and easy purification methods for total DNA.
- Ensures reliable RAP and Southern Blotting results.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Can purify DNA from diverse sources: tissu, whole blood, plasma, sérum, couche leucocyto-plaquettaire, moelle, autres fluides corporels, lymphocytes, et cellules cultivées.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total DNA from blood, tissu, culture cells, écouvillon, and blood spots using 96 plate |
| Applications | RAP, boulon du sud et détection de virus, etc. |
| Méthode de purification | 96 well plate |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Sang, sérum, plasma, milk, saliva, and other liquid samples and cultured cells |
| Montant de l'échantillon | |
| Volume d'élution | |
| Temps par course | |
| Volume de liquide transporté par colonne | |
| Rendement de liaison de la colonne |
Principe
- Utilizes silica column purification method.
- L'échantillon est lysé et digéré avec du lysat et de la protéase, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- L'acide nucléique est adsorbé sur la membrane, while protein is not adsorbed and is removed through filtration.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mmTris, pH 9.0, 0.5mmEDTA).
Avantages
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc..
- High throughput – 96 samples can be processed simultaneously
Contenu du kit
| Contenu | D311701 | D311702 |
| Temps de purification | 1 X 96 | 4 X 96 |
| HiPure gDNA Plate | 1 | 4 |
| 96 well Plate (2.2ml) | 1 | 4 |
| 1.6ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| Silicon Seal Tape | 1 | 4 |
| Seal Film | 5 | 25 |
| Tampon ATL | 30 ml | 100 ml |
| Tampon AL | 30 ml | 100 ml |
| Buffer DW1 | 60 ml | 250 ml |
| Tampon GW2 | 50 ml | 2 X 100 ml |
| Protéinase K | 50 ml | 200 ml |
| Tampon de dissolution de protéase | 5 ml | 15 ml |
| Tampon AE | 30 ml | 120 ml |
Stockage et stabilité
- Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect its performance.
- Les composants restants du kit peuvent être conservés à température ambiante (15-25°C) and remain stable for at least 18 mois dans ces conditions.
Guide d'achat
| Nom | CAT NO | Montant de l'échantillon | Leukocyte protocol* | Colum type | Volume d'élution | Average yield | Temps par course |
| Mini kit d'ADN sanguin HiPure | D3111 | 10-200µl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| Tissu HiPure&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Tissu HiPure&Kit Maxi ADN Sanguin | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Tissu HiPure&ADN sanguin 96 Trousse | D3117 | 1-200µl | 1ml | 96 well plate | 3-8μg/200μl |
Commentaires
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