- Challenges in DNA Extraction from Blood Samples:
- High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
- Complex DNA Source: Blood DNA contains various types such as mitochondrial, génomique, and circulating DNA, leading to potential loss during extraction.
- Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
- Existing Extraction Methods:
- Phenol-chloroform extraction, salting out, etc..
- These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
- Product Benefits:
- Fast and Easy Purification: Provides a rapid and straightforward method for total Purification de l'ADN.
- Suitability: Purifies total DNA (génomique, viral, mitochondriale) from whole blood, tissu, et cellules cultivées.
- Application: Ensures reliable DNA for RAP and Southern blotting assays.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation of total DNA from 10ml blood and 1g tissue using Maxi column |
| Applications | RAP, boulon du sud et détection de virus, etc. |
| Méthode de purification | Maxi spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Tissu, cellules, sang, saliva, swabs, blood spots, semen, and other clinical samples |
| Montant de l'échantillon | 3-10ml |
| Volume d'élution | ≥700μl |
| Temps par course | ≤90 minutes |
| Volume de liquide transporté par colonne | 4ml |
| Rendement de liaison de la colonne | 5mg |
Principe
- Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
- Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
- Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
- DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.
Avantages
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc..
- Rapide – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
- Large applicabilité – handle a variety of liquid samples
- Économie – excellent cost-effectiveness performance
Contenu du kit
| Contents10 | 10 préparations | 50 perps |
| Temps de purification | 10 | 50 |
| HiPure gDNA Maxi Columns | 10 | 50 |
| 50Tubes de prélèvement ml | 20 | 100 |
| Tampon ATL | 120 ml | 550 ml |
| Tampon AL | 120 ml | 2 X 300 ml |
| Tampon GW1* | 53 ml | 220 ml |
| Tampon GW2* | 25 ml | 110 ml |
| RNase A | 40 mg | 180 ml |
| Protéinase K | 120 mg | 540 mg |
| Tampon de dissolution de protéase | 12 ml | 50 ml |
| Tampon AE | 30 ml | 120 ml |
Stockage et stabilité
Storage Recommendations:
- Proteinase K and RNase A: Upon arrival, store at 2-8°C. Short-term storage (jusqu'à 12 semaines) à température ambiante (15-25°C) is acceptable without affecting performance.
- Remaining Kit Components: Can be stored at room temperature (15-25°C) and remain stable for at least 18 mois dans ces conditions.
Guide d'achat
| Nom | CAT NO | Montant de l'échantillon | Leukocyte protocol* | Colum type | Volume d'élution | Average yield | Temps par course |
| Mini kit d'ADN sanguin HiPure | D3111 | 10-200µl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| Tissu HiPure&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Tissu HiPure&Kit Maxi ADN Sanguin | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Tissu HiPure&ADN sanguin 96 Trousse | D3117 | 1-200µl | 1ml | 96 well plate | 3-8μg/200μl |
