Présentation du produit
- Offers fast and easy purification methods for total DNA.
- Ensures reliable RAP and Southern Blotting results.
- Suitable for purification of various types of DNA: génomique, viral, mitochondriale.
- Can purify DNA from tissue and cultured cells.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| Applications | RAP, boulon du sud et détection de virus, etc. |
| Méthode de purification | Midi colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Tissu, cellules, sang, saliva, swabs, blood spots, semen and other clinical samples |
| Montant de l'échantillon | 0.2-2 ml |
| Volume d'élution | ≥300μl |
| Temps par course | ≤80 minutes |
| Volume de liquide transporté par colonne | 4ml |
| Rendement de liaison de la colonne | 1mg |
Principles
- Utilizes silica column purification method.
- L'échantillon est lysé et digéré avec du lysat et de la protéase, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- L'acide nucléique est adsorbé sur la membrane, while protein is not adsorbed and is removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mmTris, pH 9.0, 0.5mmEDTA).
Avantages
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc..
- Rapide – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Large applicabilité – handle a variety of liquid samples
Contenu du kit
| Contenu | D311302 | D311303 |
| Temps de purification | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15Tubes de prélèvement ml | 40 | 200 |
| Tampon ATL | 50 ml | 250 ml |
| Tampon AL | 50 ml | 250 ml |
| Tampon GW1* | 22 ml | 110 ml |
| Tampon GW2* | 12 ml | 50 ml |
| RNase A | 20 mg | 90 mg |
| Protéinase K | 100 mg | 440 mg |
| Tampon de dissolution de protéase | 10 ml | 30 ml |
| Tampon AE | 20 ml | 120 ml |
Stockage et stabilité
- Proteinase K and RNase A should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) pour jusqu'à 12 weeks does not affect their performance.
- Les composants restants du kit peuvent être conservés à température ambiante (15-25°C) and remain stable for at least 18 mois dans ces conditions.
Données d'expérimentation
Guide d'achat
| Nom | CAT NO | Montant de l'échantillon | Leukocyte protocol* | Colum type | Elutio volume | Average yield | Temps par course |
| Mini kit d'ADN sanguin HiPure | D3111 | 10-200µl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| Tissu HiPure&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Tissu HiPure&Kit Maxi ADN Sanguin | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Tissu HiPure&ADN sanguin 96 Trousse | D3117 | 1-200µl | 1ml | 96 well plate | 3-8μg/200μl |
Commentaires
Il n'y a pas encore de critiques.