This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cellules, sang, s and other clinical samples. RNA can be used directly for RT-RAP, quantitative RT-PCR, test of virus DNA and so on.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Des produits | ARN, miARN |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Clinical tissues, cellules, lymphocytes |
| Montant de l'échantillon | Tissu: <20 mg Cells: <5 X 106 |
| Yield | 2-50µg |
| Volume d'élution | ≥30μl |
| Temps par course | ≤25 minutes |
Principe
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. Après avoir lavé les protéines et autres impuretés, RNA was finally eluted with low-saltbuffer.
Avantages
- Efficient DNA removal – one step Extraction d'ARN can effectively remove genomic DNA
- High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Rapide – the whole extraction only takes 15-25 minutes
- Nontoxic – no toxic phenol chloroform extraction is required in the extraction
Contenu du kit
| Contenu | DIV4121 |
| Temps de purification | 50 Préparations |
| HiPure DNA Mini Column Ⅱ | 50 |
| HiPure RNA Mini Columns | 50 |
| 2Tubes de prélèvement ml | 150 |
| Protéinase K | 24 mg |
| Tampon de dissolution de protéase | 1.8 ml |
| DNase I | 600 µl |
| DNase Buffer | 6 ml |
| Buffer RTL | 40 ml |
| RNA Digestion Buffer | 15 ml |
| Buffer RWC* | 20 ml |
| Buffer RW2* | 20 ml |
| Eau sans nucléase | 10 ml |
Stockage et stabilité
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Cependant, stockage à court terme (DNase I up to 1 semaines, Proteinase K up to 8 semaines) à température ambiante (15–25°C) n'affecte pas leurs performances. Les composants restants du kit peuvent être conservés à température ambiante (15–25°C) et sont stables pendant au moins 18 mois dans ces conditions.
Commentaires
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