The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissus, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-RAP, Transfert du Nord, poly A+ RNA (ARNm) purification, nuclease protection, and in vitro translation.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolement de l'ARN total (not include miRNA) from animal tissues, cells and simple plant tissues using one column |
| Applications | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Animal soft tissue, cultured cells, lymphocytes, simple plant tissue |
| Montant de l'échantillon | Cells: ≤1 x 107 Animal tissue: 1-20 mg Plant leaves: 50-150 mg Yeast cells: 5 X 106 |
| Yield | 2-100µg |
| Volume d'élution | ≥50μl |
| Temps par course | ≤15 minutes（1-24 samples） |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Avantages
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Rapide – several samples can be extracted in 20 minutes by column method
- Safe – no phenol chloroform extraction required
- Sensitive – RNA can be recovered at the level of PG
Contenu du kit
| Contenu | R401102 | R401103 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Mini Columns | 50 | 250 |
| 2Tubes de prélèvement ml | 50 | 250 |
| RTL Lysis Buffer | 50 ml | 200 ml |
| RNA Binding Buffer | 15 ml | 75 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 2 X 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Stockage et stabilité
The Kit can be stored dry at room temperature (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions.
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