Présentation du produit
- Suitable for rapid extraction of total DNA from various sources: tissu, cellules, sang, saliva, swabs, blood spots, semen, and other clinical samples.
- Extracted DNA can be used directly for RAP, quantitative PCR, Southern Blot, virus DNA testing, et d'autres applications.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| Applications | RAP, qPCR, boulon du sud et détection de virus, etc. |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Tissu, cellules, sang, saliva, swabs, blood spots, semen, and other clinical samples |
| Montant de l'échantillon | Solid tissue: 1-10mg; Sang anticoagulant: 200µl |
| Volume d'élution | ≥20μl |
| Temps par course | 30 – 60 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
- Utilizes silica column purification method.
- L'échantillon est lysé et digéré avec du lysat et de la protéase, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- L'acide nucléique est adsorbé sur la membrane, while protein is not adsorbed and removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mmTris, pH 9.0, 0.5mmEDTA).
Avantages
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc..
- Rapide – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Large applicabilité- handle a variety of liquid samples
Contenu du kit
| Contenu | D301802 | D301803 |
| Temps de purification | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2Tubes de prélèvement ml | 100 | 500 |
| Tampon ATL | 30 ml | 150 ml |
| Tampon AL | 30 ml | 150 ml |
| Tampon GW1 | 22 ml | 88 ml |
| Tampon GW2 | 12 ml | 50 ml |
| Protéinase K | 24 mg | 120 mg |
| Tampon de dissolution de protéase | 1.8 ml | 10 ml |
| Tampon AE | 15 ml | 60 ml |
Stockage et stabilité
- Proteinase K should initially be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect its performance.
- Les composants restants du kit peuvent être conservés à température ambiante (15-25°C) and remain stable for at least 18 mois.
- Alternativement, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
Données d'expérimentation
Commentaires
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