Introduction
The Kit integrates phenol/guanidine-based lysis and silica-membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate the lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg of animal, plant, and fungal samples, bactéries, cellules, and other samples.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation of total RNA (y compris les miARN) from 100mg lipid tissue, tissu, cellule, plant, and body fluids using columns and MagZol reagent |
| Applications | RT-RAP, qRT-PCR, Northern hybridization, second-generation sequencing |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice, acid phenol/guanidine extraction technology (MagZol pretreatment technology) |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Animal tissue, muscle fiber-containing tissue, adipose tissue, cultured cells, lymphocytes, simple plants, and other biological samples |
| Montant de l'échantillon | Animal tissue sample: 1-60mg (spleen ≤ 10mg) Cultured cells: 5 X 106 Plant leaves: 50-100mg |
| Yield | 2-200µg |
| Volume d'élution | ≥50μl |
| Temps par course | ≤30 minutes（1-24 samples） |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 100µg |
Principe
Tissue samples (10-100mg) are homogenized in MagZol Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.
Avantages
- Efficient DNA removal – One-step Extraction d'ARN can effectively remove genomic DNA
- High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Rapide – several samples can be extracted in 30 minutes
- High applicability – samples including animals, plantes, bactéries, cellules, etc..
- High output – enables to processing of large samples and the yield can be up to 200μg
Contenu du kit
| Contenu | R413002 | D413003 |
| Temps de purification | 50 Préparations | 250 Préparations |
| HiPure RNA Mini Columns | 50 | 250 |
| 2Tubes de prélèvement ml | 50 | 250 |
| MagZol Reagent | 60 ml | 270 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Stockage et stabilité
MagZol Reagent should be stored at 2-8°C upon arrival. Cependant, stockage à court terme (jusqu'à 12 semaines) à température ambiante (15-25°C) does not affect its performance. Les composants restants du kit peuvent être conservés à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions.
Données d'expérimentation
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