Présentation du produit
This product offers a rapid and efficient method to isolate DNA from a wide range of samples, y compris:
- Tissues
- Cells
- Sang
- Saliva
- Swabs
- Blood Spots
- Semen
- And more!
The extracted DNA is ready for immediate use in downstream applications like:
- RAP (Réaction en chaîne par polymérase)
- qPCR (quantitative PCR)
- Southern Blotting
- Viral DNA detection
- Other DNA-based analyses
spécification
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation of total DNA from tissue/blood /body fluid /swab /dry blood spots |
| Applications | RAP,qPCR, boulon du sud et détection de virus, etc.. |
| Méthode de purification | Mini spin column |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Tissu, cellules, sang, saliva, swabs, blood spots, semen, and other clinical samples |
| Montant de l'échantillon | Solid tissue:1-10mg, Sang anticoagulant:200m |
| Yield | 1 -15µg |
| Volume d'élution | ≥20μl |
| Temps par course | 30 -60 minutes |
| Volume de liquide transporté par colonne | 750µl |
| Rendement de liaison de la colonne | 100µg |
Principe
- Lysis and Digestion: Begin by lysing and digesting the sample using a specialized lysate and protease combination. This step facilitates the release of DNA into the lysate, preparing it for the purification process.
- Adsorption Column: Transfer the lysate mixture to an adsorption column. Ici, the nucleic acid selectively adheres to the silica membrane, while proteins and other contaminants remain unadsorbed. This selective binding ensures the efficient removal of impurities.
- Filtration: Employ filtration to efficiently remove the unadsorbed proteins and other contaminants from the mixture. This step further enhances the purity of the nucleic acid sample.
- Thorough Washing: Perform a thorough washing step to eliminate any remaining proteins and impurities, ensuring the purity of the nucleic acid sample.
- Elution: Finally, elute the purified nucleic acid from the adsorption column using a low-salt buffer solution (10mmTris, pH 9.0, 0.5mmEDTA). This gentle elution process preserves the integrity of the DNA, readying it for downstream applications.
Avantages
- Versatility: Suitable for a diverse range of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, et plus. Whether you’re performing basic research or advanced molecular assays, our DNA meets your requirements.
- Efficiency: Accelerate your workflow with fast DNA extraction. Say goodbye to time-consuming processes like leukocyte separation, organic extraction, or ethanol precipitation. Our method streamlines the extraction process for swift results.
- Simplicity: Enjoy a straightforward extraction process. No complex steps or specialized equipment are required. Obtain all nucleic acids directly through simple digestion, saving you time and effort in the lab.
- Broad Applicability: Our DNA extraction method is versatile and adaptable. It can handle various sample types, including liquid samples, animal tissues, et cellules cultivées. Whether you’re working with blood, tissus, or cell cultures, our method delivers consistent results across different sample types.
Contenu du kit
| Contenu | VD3018 |
| Temps de purification | 100 |
| 2Tubes de prélèvement ml | 2×100 |
| Tampon ATL | 60 ml |
| Tampon AL | 60 m |
| Tampon GW1 | 44 m |
| Tampon GW2 | 50 m |
| Protéinase K | 60 mg |
| Tampon de dissolution de protéase | 5 ml |
| Buffer AF | 15 m |
Stockage et stabilité
- Proteinase K Storage:
- Upon arrival, store at 2-8°C.
- Short-term storage (jusqu'à 12 semaines) à température ambiante (15-25°C) is acceptable without affecting performance.
- Remaining Kit Components:
- Store at room temperature (15-25°C).
- Stable for at least 18 mois dans ces conditions.
- Entire Kit Storage:
- Can be stored at 2-8°C.
- Ensure buffers are redissolved before use if stored at this temperature.
- Buffers should be at room temperature when used.
Données d'expérimentation
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