Ce produit fournit des méthodes rapides et simples pour la purification de l'ADN total pour une RAP et transfert du sud. ADN total (par exemple., génomique, plasmid) can be purified from bacterial cultures.
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total DNA from bacterial culture |
| Applications | RAP, Southern blot et digestion enzymatique, etc.. |
| Technologie de purification | Technologie des billes magnétiques |
| Méthode de processus | Manuel ou automatique |
| Échantillon type | Gram negative or positive bacteria |
| Montant de l'échantillon | Bacterial culture: 0.5-1.5ml |
| Volume d'élution | ≥60μl |
| Temps par course | ≤60 minutes |
Principe
- This product utilizes high-binding magnetic particles for purification.
- Samples are lysed and digested using a lysate and Lysozyme.
- DNA is released into the lysate during this process.
- Magnetic particles and binding solution are added, allowing DNA to be adsorbed onto the surface of the particles while impurities such as proteins are not adsorbed.
- The adsorbed particles undergo washing with a washing solution to remove proteins and impurities.
- Ethanol is used for further washing to remove salts.
- Finally, DNA is eluted using Elution Buffer.
Avantages
- Rapide: Multiple samples can be extracted within 60 minutes (after digestion), making the process time-efficient.
- Haute pureté: Unique adsorbents ensure the complete removal of inhibitory factors, resulting in high-purity DNA extraction.
- Récupération élevée: DNA can be recovered at the level of picograms (PG), indicating a high level of efficiency in the extraction process.
Contenu du kit
| Contenu | D513101 | D513102 | D513103 |
| Temps de purification | 48 Préparations | 96 Préparations | 480 Préparations |
| Particules MagPure | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Protéinase K | 24 mg | 50 mg | 220 mg |
| Tampon de dissolution de protéase | 3 ml | 6 ml | 30 ml |
| Tampon FDS | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Tampon GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Stockage et stabilité
- Particules MagPure, Lysozyme, and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage (jusqu'à 12 semaines) à température ambiante (15-25°C) n'affecte pas leurs performances.
- Les composants restants du kit peuvent être stockés au sec à température ambiante (15-25°C) and remain stable for at least 18 mois dans ces conditions.
Commentaires
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