Introduction
This kit empowers researchers with a streamlined solution for isolating high-quality circulating cell-free DNA (cfDNA) from various biofluids:
- Sample Types: Plasma, Sérum (1-6ml)
- Target: cfDNA molecules
- Downstream Applications:
- RAP (réaction en chaîne par polymérase)
- Pcr en temps réel
- Biochip analysis
- Next-generation sequencing (NGS)
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation circulating DNA from 1-6ml plasma, sérum, fluides corporels |
| Applications | qPCR, NGS, etc.. |
| Technologie de purification | Technologie des billes magnétiques |
| Méthode de processus | Manuel ou automatique |
| Échantillon type | Sérum, plasma |
| Montant de l'échantillon | 1-6ml |
| Volume d'élution | ≥50μl |
| Temps par course | ≤60 minutes |
Principe
This product utilizes innovative magnetic bead technology for efficient DNA extraction:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
- Binding to Magnetic Beads: Magnetic particles coated for high DNA affinity are added to the lysate. DNA molecules selectively bind to the surface of these beads, while impurities like proteins remain unbound.
- Washing and Purification: A series of washes with different solutions remove unbound proteins and other cellular debris.
- Elution: A special elution buffer disrupts the interaction between the DNA and the magnetic beads, allowing the purified DNA to be collected in a separate tube.
Avantages
- Économie – moins que 50% du prix de Qiagen et d'autres produits importés
- Automatique – without labor
- Haute pureté – OD 260 / 280: 1.7-1.9, OD 260 / 230:1.5-2.0
Contenu du kit
| Contenu | IVD5435 |
| Temps de purification | 50 |
| Particules MagPure F | 14 ml |
| ARN porteur | 310 µg |
| Protéinase K | 240 mg |
| Tampon de dissolution de protéase | 15 ml |
| Tampon FDS | 15 ml |
| Tampon MLK | 500 ml |
| Buffer MAW1 | 250 ml |
| Tampon MW2* | 50 ml |
| Tampon d'élution | 60 ml |
Stockage et stabilité
Ideal Storage:
- Store Proteinase K, ARN porteur, and MagPure Particles F in the refrigerator (2-8°C) upon arrival for maximum effectiveness.
Acceptable Short-Term Storage:
- These components can be kept at room temperature (15-25°C) for up to 12 weeks without impacting their performance.
Storing Remaining Components:
- All other kit components can be stored dry at room temperature (15-25°C).
- They will remain stable for at least 18 mois dans ces conditions.
Important Notes:
- If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
- Always ensure all buffers reach room temperature before using them for optimal results.
Données d'expérimentation
Commentaires
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