This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Isolation total DNA from tissue using economic salt out method |
| Applications | RAP, enzyme digestion, Southern hybridization,etc.. |
| Technologie de purification | Salting out method |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Animal tissue |
| Montant de l'échantillon | Unlimited |
| Volume d'élution | ≥100μl |
| Temps par course | Variation with sample amount |
Principles
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, comme les protéines, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 et 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
Avantages
- Safe – without phenol chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- Haute pureté – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Contenu du kit
| Contenu | D331201 | D331202 | D331203 |
| Temps de purification | 1 g | 5 g | 50 g |
| Protéinase K | 330 µl | 1.8 ml | 18 ml |
| Buffer WTL | 33 ml | 160 ml | 2 X 800 ml |
| Buffer PPS | 12 ml | 55 ml | 500 ml |
| Solution de RNase | 330 µl | 1.8 ml | 18 ml |
| Buffer TE | 12 ml | 60 ml | 200 ml |
Stockage et stabilité
RNase Solution should be stored at 2-8°C upon arrival. Cependant, stockage à court terme (jusqu'à 24 semaines) à température ambiante (15-25°C) n'affecte pas leurs performances. Les composants restants du kit peuvent être stockés au sec à température ambiante (15-25°C) et sont stables pendant au moins 18 mois dans ces conditions. L'ensemble du kit peut être conservé entre 2 et 8°C, mais dans ce cas, les tampons doivent être redissous avant utilisation. Assurez-vous que tous les tampons sont à température ambiante lorsqu'ils sont utilisés.
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