SYBR Green 2xqPCR Mix 1ml *10

Name: SYBR Green 2xqPCR Mix 1ml *10
SKU: 210
Price: 80.00 USD
Availability: InStock

$80.00

Expédition USD 45 - Gratuit sur USD 300

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Fabricant: Principales marques chinoises
Expédition: Expédition FedEx accélérée directement depuis les usines
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Description

Quantitative RAP (qPCR) Overview:
- qPCR, also known as Real-time PCR, is a widely used technology for precise analysis of gene expression.
- It can be categorized into dye-based and probe-based methods, with dye-based being more popular, convenient, and cost-effective.
- Dye-based qPCR monitors real-time fluorescence of the dye binding to double-stranded DNA to measure DNA amplification indirectly during each cycle.
- Ct value (Cq value) is determined when the fluorescence signal is distinctly detected over the background, which can be used to evaluate target abundance or calculate absolute quantities.
SYBR Green 2xqPCR Mix Master Mix:
- Provides superior specificity, robust amplification efficiency, ideal reproducibility, and stability in quantifying target DNA or cDNA.
- It’s a 2X premix containing hot-start Taq polymérase combined with an antibody for preferable specificity and high amplification speed.
- SYBR Green I in the mix emits fluorescence when bound to double-stranded DNA amplified in each cycle, allowing for indirect quantification of amplification products in real-time.
- Use SYBR or SYBR/FAM scan mode setting on the real-time instrument.
Limitations of Dye-based qPCR:
- SYBR Green I can intercalate into any double-stranded DNA, including primer-dimers or other undesired products, causing nonspecific fluorescence.
- To confirm specificity, perform a melt curve analysis after amplification, where a sharp peak around the annealing temperature is ideal.