Uvod
Blood is a valuable source of DNA for various clinical applications and research studies. Međutim, extracting DNA from blood samples presents several challenges:
Safety Concerns:
- Blood samples can harbor pathogens, posing a risk of infection to researchers and contaminating the lab environment.
- Traditional methods often involve hazardous chemicals like phenol-chloroform.
DNA Loss:
- Removing red blood cells, a common pre-treatment step can lead to the loss of desired DNA fractions like viral DNA, circulating DNA, and microbial DNA.
Impurities and Inhibitors:
- Blood contains various components that can interfere with downstream analyses like PCR and Southern blotting.
New Technology for Improved Blood DNA Extraction:
This product offers a novel approach to address these challenges:
- Safe and Efficient: The method eliminates the need for red blood cell removal, minimizing infectious waste and potential contamination.
- Comprehensive DNA Recovery: The protocol allows for the purification of total DNA, including genomic, mitohondrijski, viral, and circulating DNA, providing a more complete picture for analysis.
- Broad Sample Compatibility: The method effectively purifies DNA from various blood components like whole blood (fresh or frozen), plasma, serum, buffy coat, and more.
- Downstream Applications: The extracted high-quality DNA is suitable for reliable PCR and Southern blotting analyses.
This innovative solution simplifies blood DNA extraction while ensuring safety, efficiency, and comprehensive DNA recovery. It represents a significant advancement for researchers working in various fields like diagnostics, forensics, and medical research.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total DNA from 200ul Whole Blood |
| Prijave | PCR, Southern Bolt i otkrivanje virusa, itd |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Whole Blood (fresh or frozen), serum, plasma, milk, saliva, and other liquid samples and cultured cells |
| Količina uzorka | <200μl whole blood or other liquid samples, <5*106 lymphocytes or Culture Cells Non-mammalian animals that have a nucleus in red blood cells (rich in DNA, such as birds and fish): 5~20μl whole blood at a time. |
| Volumen eluacije | ≥20μl |
| Vrijeme po vožnji | ≤30 minuta |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
This method provides a reliable and convenient solution for researchers working in various fields that require purified DNA for analysis, such as PCR, Southern blotting, and next-generation sequencing.
Efficient DNA Purification with Silica Column Technology
This product utilizes a silica column-based method for rapid and efficient DNA purification. Here’s a breakdown of the process:
- Sample Lysis and Digestion: The sample is treated with a lysate solution and protease to break down cell membranes and degrade proteins. This releases DNA into the solution.
- Binding to Silica Membrane: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column specifically binds DNA molecules through favorable interactions. Proteins and other impurities remain unbound.
- Washing: Wash buffers are passed through the column, removing unbound proteins and cellular debris that did not adhere to the silica membrane.
- Elution: Finally, a low-salt buffer (typically 10 mM Tris, pH 9.0, i 0.5 mM EDTA) is used to elute the purified DNA from the silica membrane. This buffer disrupts the interaction between DNA and the silica, allowing the purified DNA to be collected in a separate tube.
Prednosti
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, itd.
- Brzo – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability- handle a variety of liquid samples
Sadržaj kompleta
| Sadržaj | D311102 | D311103 |
| Vremena pročišćavanja | 50 | 250 |
| HiPure DNA mini stupci I | 50 | 2 x 125 |
| 2ml cijevi za prikupljanje | 100 | 5 x 100 |
| Buffer AL | 15 ml | 60 ml |
| Međuspremnik DW1 | 30 ml | 150 ml |
| Međuspremnik GW2* | 12 ml | 50 ml |
| Proteinaza K | 24 mg | 120 mg |
| Pufer za otapanje proteaze | 1.8 ml | 10 ml |
| Međuspremnik AE | 15 ml | 60 ml |
Skladištenje i stabilnost
For optimal performance:
- Store Proteinase K at 2-8°C (refrigerator) upon arrival.
Acceptable short-term storage:
- Proteinase K can be stored for up to 12 weeks at room temperature (15-25°C) without affecting its performance.
Remaining kit components:
- Store all other components of the kit dry at room temperature (15-25°C).
- They are stable for at least 18 mjeseci pod ovim uvjetima.
Important Note:
- If storing the entire kit at room temperature, ensure buffers are redissolved before use and always warm all buffers to room temperature before using them.
