Uvod
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA and miRNA from cell and tissue without MagZol reagent |
| Prijave | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Cell, Animal tissue, Plant tissue |
| Količina uzorka | Cells: ≤ 5 x 10^6, Animal tissue:<10mg |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | ≤40 minuta |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.
Prednosti
- High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Brzo – može se izdvojiti nekoliko uzoraka 40 minuta
- High applicability – samples including animals, plants, bacteria, cells, itd.
- High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
- Sef – no phenol chloroform extraction and no use of Trizol reagent
Sadržaj kompleta
| Sadržaj | R431102 | R431103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml cijevi za prikupljanje | 100 | 2 x 250 |
| Proteinaza K | 48 mg | 240 mg |
| Pufer za otapanje proteaze | 5 ml | 15 ml |
| Buffer RLC | 40 ml | 200 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 60 ml |
Skladištenje i stabilnost
Proteinazu K treba čuvati na 2-8°C po dolasku. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15-25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
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