Product Overview
- Offers fast and easy methods for purifying total DNA.
- Ensures reliable PCR and Southern blotting results.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Can purify DNA from small volumes of blood, tissue, and dry blood spots.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total DNA from 1-10μl blood, <10mg tissue, urine, blood stain, seminal stain |
| Prijave | PCR, Southern Bolt i otkrivanje virusa, itd. |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Animal tissues, blood stain, urine, seminal stain and various forensic samples |
| Količina uzorka | Blood:1-100μl, Tissue:<10mg |
| Volumen eluacije | |
| Vrijeme po vožnji | |
| Nosivi volumen tekućine po stupcu | |
| Prinos vezivanja kolone |
Načelo
- Utilizes silica column purification method.
- Sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nukleinska kiselina se adsorbira na membrani, dok se protein ne adsorbira i uklanja se filtracijom.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Prednosti
- Brzo – može se izdvojiti nekoliko uzoraka 20 minuta (after digestion)
- Visoka čistoća – purified DNA can be directly used in various downstream applications
- Visoki oporavak – DNA can be recovered at the level of PG
- Dobra ponovljivost – silica technology can obtain ideal results every time
Sadržaj kompleta
| Sadržaj | D312502 | D312503 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| Buffer ATL | 15 ml | 60 ml |
| Buffer AL | 15 ml | 60 ml |
| Međuspremnik GW1* | 22 ml | 66 ml |
| Međuspremnik GW2* | 20 ml | 2 x 50 ml |
| Carrier RNA | 310 μg | 2 x 310 µg |
| Proteinaza K | 24 mg | 120 mg |
| Pufer za otapanje proteaze | 1.8 ml | 10 ml |
| Međuspremnik AE | 15 ml | 60 ml |
| HiPure DNA mini stupci I | 50 | 2 x 125 |
| 2 ml cijevi za prikupljanje | 100 | 5 x 100 |
Skladištenje i stabilnost
- Carrier RNA and Proteinase K should be stored at 2–8°C upon arrival.
- Short-term storage at room temperature (15–25°C) for up to 12 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15–25°C) and remain stable for at least 18 mjeseci pod ovim uvjetima.
Experiment Data
Recenzije
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