Uvod
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample) |
| Prijave | SDS-PAGE electrophoresis and western blot, itd |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples |
| Količina uzorka | Cultured cells: < 10^7 Animal tissue: ≤ 20 mg Plant samples: ≤ 150 mg Yeast cells: 2 x 10^6 – 5 x 10^7 |
Načelo
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
Prednosti
- High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
- Brzo – column method can complete the extraction of several samples in 30 minuta
- Sef – no phenol chloroform extraction required
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Sadržaj kompleta
| Sadržaj | R521102 | R521103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml cijevi za prikupljanje | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Međuspremnik GW1* | 22 ml | 66 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 50 ml | 3 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
| Elucijski pufer | 10 ml | 30 ml |
| Buffer ALO(5%SDS) | 10 ml | 30 ml |
Skladištenje i stabilnost
HiPure Kit can be stored dry at room temperature (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Recenzije
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