This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation gDNA from biological sample and remove host DNA |
| Prijave | PCR, southern blot and enzyme digestion, itd. |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Culture medium, swab, parasitic blood, tissue, sputum, itd. |
| Količina uzorka | Blood sample, homogenate, plasma, brain effusion, swab immersion solution, centrifuged concentrated liquid, itd.:0.5-1ml |
| Volumen eluacije | ≥50μl |
| Vrijeme po vožnji | ≤60 minuta |
| Nosivi volumen tekućine po stupcu | 800μl |
| Prinos vezivanja kolone | 100μg |
Načelo
Ovaj proizvod se temelji na pročišćavanju kolonom silicijevog dioksida. This product efficiently depletes human and animal host DNA and yields enriched bacterial DNA. An optimized combination of mechanical and chemical lysis allows efficient disruption of bacterial cells. Target DNA is adsorbed on the membrane, dok se protein ne adsorbira i uklanja se filtracijom. Nakon pranja proteina i drugih nečistoća, nukleinska kiselina je konačno eluirana s puferom s malo soli (10mm Tris, pH 9,0, 0.5mm EDTA).
Prednosti
- High concentration – the host DNA is digested by dnase, without contamination of host DNA
- Visoki oporavak – DNA can be recovered at the level of PG
- Dobra ponovljivost – silica technology can obtain ideal results every time
- Wide applicability – it can be used in blood, tissue, intestinal contents and other samples
Sadržaj kompleta
| Sadržaj | D314802 | D314803 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| Hipure DNA Mini Columns I | 50 | 2 x 125 |
| 2ml cijevi za prikupljanje | 50 | 2 x 125 |
| 2ml beads Tubes | 50 | 250 |
| Buffer DRB | 15 ml | 60 ml |
| Buffer ES | 6 ml | 30 ml |
| Reagent DX | 0.5 ml | 1 ml |
| Buffer DL | 30 ml | 120 ml |
| Buffer GW1 | 22 ml | 110 ml |
| Međuspremnik GW2 | 12 ml | 50 ml |
| DNase I (Powder) | 6 mg | 30 mg |
| Proteinaza K | 24 mg | 120 mg |
| Pufer za otapanje proteaze | 5 ml | 15 ml |
| Međuspremnik AE | 15 ml | 60 ml |
Skladištenje i stabilnost
DNase I and Proteinase K should be stored at 2–8°C upon arrival. Međutim, kratkotrajno skladištenje (do 12 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na suhom na sobnoj temperaturi (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Cijeli komplet može se čuvati na 2–8°C, ali u ovom slučaju pufere treba ponovno otopiti prije upotrebe. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste.
Recenzije
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