The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total RNA from Paxgene RNA Tubes |
| Prijave | RT-PCR, qPCR, Northern hybridization, NGS, nucleic acid protection, in vitro translation |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija, DNA filtration technology |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Blood in the preservation tube |
| Količina uzorka | 2.5ml |
| Volumen eluacije | ≥30μl |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100μg |
Načelo
The Kit is for the purification of total RNA from 2.5 ml human whole blood collected in a PAXgene Blood RNA Tube. Purification begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed and resuspended, and incubated in optimized buffers together with proteinase K. DNA wash The lysate is passed through a DNA Mini column. Ethanol is added to adjust binding conditions, and the lysate is applied to a column.RNA is selectively bound to the silica membrane as contaminants pass through. The remaining contaminants are removed in several efficient wash steps. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA is eluted in elution buffer and heat-denatured.
Prednosti
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Brzo – isolation of several samples can be completed in 40 minutes by using the column purification method
- Safety – no phenol-chloroform extraction is required
- Sensitive – direct lysis of blood, plasma, and other samples without separation of leukocytes
Sadržaj kompleta
| Sadržaj | R416802 | R416803 |
| Vremena pročišćavanja | 10 Pripreme | 50 Pripreme |
| HiPure RNA Mini Columns I | 10 | 50 |
| gDNA Filter Mini Columns | 10 | 50 |
| 2ml cijevi za prikupljanje | 30 | 150 |
| RNase Free Water | 60 ml | 250 ml |
| Buffer MBR1 | 10 ml | 30 ml |
| Buffer MBR2 | 5 ml | 15 ml |
| Buffer RW1 | 15 ml | 60 ml |
| Buffer RW2* | 6 ml | 20 ml |
| Proteinaza K | 12 mg | 50 mg |
| Pufer za otapanje proteaze | 1.8 ml | 5 ml |
| DNase I | 120 µl | 600 µl |
| DNase Buffer | 6 ml | 30 ml |
Skladištenje i stabilnost
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Međutim, kratkotrajno skladištenje (DNase I up to 1 tjedni, Proteinase K up to 8 tjedni) na sobnoj temperaturi (15–25°C) ne utječe na njihovu izvedbu. Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Experiment Data
Recenzije
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