The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
pojedinosti
Tehnički podaci
Značajke | Tehnički podaci |
Glavne funkcije | Isolation total RNA from <150 mg simple plant sample (including plant sample containing starch) without chloroform |
Prijave | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
Metoda pročišćavanja | Mini spin column |
Tehnologija pročišćavanja | Silika tehnologija, DNA filtration technology |
Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
Vrsta uzorka | Economic crops |
Količina uzorka | ≤150 mg |
Volumen eluacije | ≥30μl |
Vrijeme po vožnji | ≤25 minutes |
Nosivi volumen tekućine po stupcu | 800µl |
Prinos vezivanja kolone | 100µg |
Načelo
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanolis added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Prednosti
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Brzo – može se izdvojiti nekoliko uzoraka 25 minutes by column method
- Sef – no phenol chloroform extraction required
- Sensitive – RNA can be recovered at the level of PG
- Široki spektar – various types of plant samples can be processed by diversity of operating procedures
Sadržaj kompleta
Sadržaj | R415102 | D415103 |
Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
HiPure DNA Mini Column II | 50 | 250 |
HiPure RNA Mini Columns | 50 | 250 |
2ml cijevi za prikupljanje | 100 | 2 x 250 |
Buffer RLC | 50 ml | 200 ml |
Buffer PRC1 | 50 ml | 200 ml |
Buffer RW1 | 50 ml | 200 ml |
Buffer RW2* | 20 ml | 2 x 50 ml |
RNase Free Water | 10 ml | 30 ml |
Skladištenje i stabilnost
The Kit can be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Provjerite jesu li svi puferi na sobnoj temperaturi kada se koriste. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Recenzije
Još nema recenzija.