Uvod
HiPure Plasmid EF Maxi Kit Features:
- Technology Integration:
- The kit integrates HiPure technology for plasmid purification with Magen’s innovative Endotoxin Removal Technology (ETR).
- Endotoxin Removal:
- Specifically designed to achieve low levels of endotoxins in the purified plasmid DNA, enhancing its suitability for sensitive applications.
- Application Suitability:
- Ideal for use in eukaryotic transfection and various in vitro experiments due to the high purity and low endotoxin levels of the DNA.
- Capacity and Yield:
- Capable of purifying up to 1000 µg of high copy number plasmid DNA or 200 µg of low copy number plasmid DNA.
- Culture Volume Processing:
- Efficient purification from up to 150 mL of an overnight bacterial culture.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of up to 1.5 mg endotoxin-free plasmid DNA from 200ml bacterial culture |
| Prijave | Transfekcija stanica, injekcija životinja, itd. |
| Metoda pročišćavanja | Maxi spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Konvencionalni plazmid, large plasmid |
| Količina uzorka | High copy plasmid: 100-150ml culture medium Low copy plasmid: 150-200ml culture medium |
| Prinos | 100-1500μg |
| Volumen eluacije | ≥0,8 ml |
| Vrijeme po vožnji | ≤60 minuta |
| Nosivi volumen tekućine po stupcu | 20ml |
| Prinos vezivanja kolone | 1 mg |
Načelo
HiPure Plasmid Procedure:
- Alkaline Lysis and DNA Adsorption:
- Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
- The unique silica membrane in the kit replaces glass or silica slurries for plasmid DNA minipreps.
- Three Basic Steps:
- Step 1: Preparation and clearing of a bacterial lysate using the alkaline method.
- Step 2: Transfer of the supernatant to a column for DNA binding.
- Step 3: Washing to remove proteins and other impurities, followed by elution of nucleic acid with low-salt buffer (10 mm Tris, pH 9.0, 0.5 mm EDTA).
Prednosti
- High purity — purified plasmid can be directly used in sequencing, enzimska probava i PCR, itd.
- Fast — silica gel column purification is much faster than ion exchange
- High yield — up to 1.5 mg plasmid can be binded in one column
- Ultra low endotoxin — content of endotoxin is less than 0.05 EU/μg, which can be directly used for cell transfection and animal injection, itd.
Sadržaj kompleta
| Sadržaj | P115602 | P115603 |
| Vremena pročišćavanja | 10 Pripreme | 50 Pripreme |
| RNaza A | 30 mg | 150 mg |
| Buffer P1 | 100 ml | 500 ml |
| Buffer P2 | 100 ml | 500 ml |
| Buffer LN3 | 50 ml | 250 ml |
| Buffer PW1 | 33 ml | 180 ml |
| Buffer PW2 | 20 ml | 100 ml |
| Elucijski pufer | 15 ml | 120 ml |
| Buffer CL | 33 ml | 180 ml |
| HiPure DNA Maxi Columns C | 10 | 50 |
| Lysate Clear Midi štrcaljka | 10 | 50 |
| 50 ml cijevi za prikupljanje, C | 20 | 100 |
Skladištenje i stabilnost
Storage Instructions:
- General Storage:
- Kit components can be stored dry at room temperature (15–25 °C).
- Stable for at least 18 mjeseci pod ovim uvjetima.
- Buffer Precipitates:
- Ako se u puferima stvori bilo kakav talog, warm at 37°C to dissolve.
- Specific Storage for RNase A and Buffer P1:
- RNase A and Buffer P1 are stable for 6 months when stored at 2–8 °C.
Vodič za kupnju
| Ime | MAČKA BR | Količina uzorka | Vrsta stupca | Volumen eluacije | Prinos | Vrijeme po vožnji | Zahtjevi za centrifugu |
| HiPure Plasmid EF Mini Kit | P1154 | 10-15ml | 1.5ml stupca | ≥75μl | 10–70µg/15 ml | ≤40 minuta | mala centrifuga |
| HiPure plazmid EF 96 Kit | P1157 | 1-5ml | 96 bunar ploča | ≥75μl | 30µg/1 ml | ≤60 minuta | 96 well plate centrifuge |
| HiPure Plasmid EF Midi Kit | P1231 | 30-50ml | 15ml stupca | ≥0,4 ml | 10-500μg/50 ml | ≤60 minuta | 15ml centrifuga |
| HiPure Plasmid EF Maxi Kit | P1156 | 100-200ml | 50ml stupca | ≥0,8 ml | 100–1500µg/200ml | ≤60 minuta | 50ml centrifuga |
| HiPure Plasmid EF Mega Kit | P1116 | 500ml | 50ml stupca | ≥0,8 ml | 10mg/500 ml | ≤70 minuta | 50ml centrifuga |
Recenzije
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