Uvod
- HiPure Plazmid DNA Plus 96 Kit:
- Enables up to 96 mini-preps to be performed simultaneously.
- Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96).
- Provides a fast, jednostavan, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications.
- Key Features:
- Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries.
- Plasmid DNA purified with the kit is immediately ready for use.
- Eliminates the need for phenol extraction and ethanol precipitation.
- High-quality plasmid DNA is eluted in a small volume of Tris buffer or water.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate |
| Prijave | Enzyme digestion, sequencing, PCR, labeling, itd. |
| Metoda pročišćavanja | 96 bunar ploča |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Konvencionalni plazmid, plazmid manji od 30KB |
| Količina uzorka | 1-1.5ml（x96） |
| Prinos | 1-15µg/1 ml |
| Volumen eluacije | ≥70μl |
| Vrijeme po vožnji | ≤60 minuta |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 70µg |
Načelo
- HiPure Plasmid Procedure:
- Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
- Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps.
- Procedure Steps:
- Preparation and Clearing of Bacterial Lysate:
- The alkaline method is used to prepare and clear bacterial lysate.
- DNA Binding:
- Supernatant transferred to a column to bind DNA to the silica membrane.
- Washing and Elution:
- Proteins and other impurities washed away.
- Nukleinska kiselina je konačno eluirana puferom s malo soli (10mm Tris, pH 9,0, 0.5mm EDTA).
- Preparation and Clearing of Bacterial Lysate:
Prednosti
- Visoka čistoća – pročišćeni plazmid može se izravno koristiti u sekvenciranju, enzyme digestion PCR, itd.
- Brzo – it takes only 60 minutes to complete the isolation
- Visok prinos – up to 1mg plasmid can be bound in one column
- Economy – high cost-performance
Sadržaj kompleta
| Sadržaj | P100601 | P100602 | P100603 |
| Vremena pročišćavanja | 1 x 96 Pripreme | 4 x 96 Pripreme | 20 x 96 Pripreme |
| RNaza A | 5 mg | 20 mg | 100 mg |
| Buffer P1 | 30 ml | 120 ml | 600 ml |
| Buffer P2 | 30 ml | 120 ml | 600 ml |
| Buffer P3 | 40 ml | 180 ml | 800 ml |
| Buffer PW1 | 100 ml | 500 ml | 2 x 1000 ml |
| Buffer PW2 | 50 ml | 2 x 100 ml | 4 x 200 ml |
| Elucijski pufer | 150 ml | 60 ml | 300 ml |
| Lysate Clear Plate | 1 | 4 | 20 |
| HiPure DNA Plate | 1 | 4 | 20 |
| 1.6 ml Collection Plate | 1 | 4 | 20 |
| 0.5ml Elute Plate | 1 | 4 | 20 |
Skladištenje i stabilnost
Komponente kompleta mogu se čuvati na suhom na sobnoj temperaturi (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima. Ako se u puferima stvori bilo kakav talog, zagrijte na 37 ℃ da se otopi. After the addition of RNase A， Međuspremnik P1 je stabilan za 6 months when stored at 2-8°C.
Vodič za kupnju
| Ime | MAČKA BR | Količina uzorka | Vrsta stupca | Volumen eluacije | Prinos | Vrijeme po vožnji | Zahtjevi za centrifugu |
| HiPure Plasmid Mini Kit | P1001 | 1-5ml | 1.5ml stupca | 30-50μl | 5–35µg/5ml | ≤30 minuta | mala centrifuga |
| HiPure Plasmid Mini Kit II | P1002 | 5-15ml | 1.5ml stupca | 75-100μl | 20-80µg/15ml | ≤30 minuta | mala centrifuga |
| HiPure Plasmid Plus 96 Kit | P1006 | 1-1.5ml | 96 bunar ploča | 70-150μl | 1-15μg/1ml | ≤60 minuta | 96-well plate centrifuge |
| HiPure Fastfilter Plasmid Midi Kit | P1013 | 30-50ml | 15ml stupca | 0.3-0.8ml | 50–250µg/50ml | ≤60 minuta | 15ml centrifuga |
| HiPure Fastfilter Plasmid Maxi Kit | P1014 | 100-200ml | 50ml stupca | 0.5-1.5ml | 0.4–1mg/200ml | ≤60 minuta | 50ml centrifuga |
Recenzije
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