Uvod
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation total RNA and miRNA from 0.2-0.6ml serum and plasma |
| Prijave | |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | |
| Količina uzorka | |
| Prinos | |
| Volumen eluacije | |
| Vrijeme po vožnji | |
| Nosivi volumen tekućine po stupcu | |
| Prinos vezivanja kolone |
Načelo
HiPure RNA technology simplifies total RNA isolation. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After the removal of protein the binding conditions for nucleic acids are adjusted by adding isopropanol. Total nucleic acids are bound to the column. Optionally, DNA can be removed by an on-column DNase digest. The remaining nucleic acids are washed and eluted with minimal amounts of RNase-Free water.
Sadržaj kompleta
| Sadržaj | R431402 | R431403 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml cijevi za prikupljanje | 100 | 2 x 250 |
| Buffer CFL | 6 ml | 30 ml |
| Buffer CFP | 1.8 ml | 10 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 60 ml |
Skladištenje i stabilnost
The kit components can be stored at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
Recenzije
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