HiPure Soil/Stool DNA 96 Kit

$597.00$8,951.00

Dostava USD 45 - Besplatno preko USD 300

DTE je kineska platforma za e-trgovinu specijalizirana za online prodaju molekularnog testiranja, ELISA, i srodni proizvodi.

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Opis

  • Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
  • Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 minuta.
  • Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology.
  • Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
  • Suitability for Various Applications: The purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
  • Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
  • Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.

Tehnički podaci

ZnačajkeTehnički podaci
Glavne funkcijeIsolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate
PrijavePCR, southern blot and enzyme digestion, itd.
Metoda pročišćavanja96 bunar ploča
Tehnologija pročišćavanjaSilika tehnologija
Procesna metodaPriručnik (centrifugiranje ili vakuum)
Vrsta uzorkaSoil, stool, other environmental samples
Količina uzorkaSoil: 200-500mg

Stool: 50-100mg

Other environmental samples: 100-500mg

Volumen eluacije
Vrijeme po vožnji
Nosivi volumen tekućine po stupcu
Prinos vezivanja kolone

Načelo

  • Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, yeast, and fungal samples.
  • Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, proteins, polysaccharides, and other contaminants from the lysed sample.
  • DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
  • Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
  • Elution of Purified DNA: Pure DNA is eluted from the column in a low ionic strength buffer, providing high-quality, purified DNA ready for downstream applications.
  • Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.

Prednosti

  • Brzo – može se izdvojiti nekoliko uzoraka 40 minuta (after digestion)
  • Visoka čistoća – the purified DNA can be directly used in various downstream applications
  • Dobra ponovljivost – silica technology can obtain ideal results every time
  • Visoki oporavak – DNA can be recovered at the level of PG

Sadržaj kompleta

SadržajD314401D314402D314403
Vremena pročišćavanja1 x 96 Pripreme4 x 96 Pripreme20 x 96 Pripreme
HiPure DNA Plate1420
96 Well Plate (2.2ml)1420
1.6ml Collection Plate1420
0.8ml Collection Plate1420
2ml Bead Tubes1004002000
Buffer SOL100 ml360 ml2 x 900 ml
Buffer SDS10 ml36 ml180 ml
Reagent DX1 ml1.8 ml9 ml
Buffer PS20 ml80 ml400 ml
Absorber Solution20 ml80 ml400 ml
Buffer GDP150 ml500 ml3 x 900 ml
Međuspremnik GW2*50 ml100 ml4 x 200 ml
Međuspremnik AE30 ml120 ml500 ml

Skladištenje i stabilnost

  • Absorber Solution Storage: Upon arrival, the Absorber Solution should be stored at 2-8°C. Međutim, it can also be stored at room temperature (15-25°C) for short-term periods (do 24 tjedni) without affecting its performance.
  • Remaining Kit Components: The remaining components of the kit can be stored dry at room temperature (15-25°C). They remain stable for at least 18 mjeseci pod ovim uvjetima, ensuring their efficacy and performance over an extended period.

Dodatne informacije

Težina0.65 kg
veličina

HiPure Soil/Stool DNA 96Kit 1 x 96preps, HiPure Soil/Stool DNA 96Kit 4 x 96preps, HiPure Soil/Stool DNA 96Kit 20 x 96preps

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