Product Overview
- Offers fast and easy methods for purification of total DNA.
- Ensures reliable PCR and Southern blotting.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Suitable for purification from tissue and cultured cells.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total DNA from <25 mg tissue, culture cells, FTA card |
| Prijave | PCR, Southern Bolt virus detection, itd. |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Animal tissue or cultured cells |
| Količina uzorka | Animal tissue : <25mg, Cultured cells : <5 x 106 |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | 30 – 60 minuta |
| Nosivi volumen tekućine po stupcu | 800μl |
| Prinos vezivanja kolone | 100μg |
Načelo
- Utilizes silica column purification method.
- Uzorak se lizira i probavlja lizatom i proteazom, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nukleinska kiselina se adsorbira na membrani, dok se protein ne adsorbira i uklanja se filtracijom.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Prednosti
- Dobra ponovljivost – suitable for extracting high-yield DNA from different types of tissue samples
- Visoka čistoća – can be used for downstream applications such as multiplex and quantitative PCR
- Fas t- simplified process, extracting several samples in 20 minuta (after digestion)
- Safety – no phenol or chloroform extraction, no alcohol precipitation
Sadržaj kompleta
| Sadržaj | D312102 | D312103 |
| Vremena pročišćavanja | 50 Pripreme | 250 Pripreme |
| Buffer ATL | 15 ml | 65 ml |
| Buffer DL | 15 ml | 65 ml |
| Buffer GW1 | 22 ml | 110 ml |
| Međuspremnik GW2 | 12 ml | 50 ml |
| RNaza A | 10 mg | 50 mg |
| Proteinaza K | 24 mg | 120 mg |
| Pufer za otapanje proteaze | 5 ml | 15 ml |
| Međuspremnik AE | 15 ml | 60 ml |
| HiPure gDNA Mini Columns | 50 | 2 x 125 |
| 2 ml cijevi za prikupljanje | 100 | 5 x 100 |
Skladištenje i stabilnost
- RNase A and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 24 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15-25°C) i stabilni su najmanje 18 mjeseci pod ovim uvjetima.
- Alternatively, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
Recenzije
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