Product Overview
- Offers fast and easy purification methods for total DNA.
- Ensures reliable PCR and Southern Blotting results.
- Suitable for purification of various types of DNA: genomski, viral, mitohondrijski.
- Can purify DNA from tissue and cultured cells.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| Prijave | PCR, Southern Bolt i otkrivanje virusa, itd |
| Metoda pročišćavanja | Midi spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Tkivo, cells, krv, saliva, swabs, blood spots, semen and other clinical samples |
| Količina uzorka | 0.2-2 ml |
| Volumen eluacije | ≥300μl |
| Vrijeme po vožnji | ≤80 minutes |
| Nosivi volumen tekućine po stupcu | 4ml |
| Prinos vezivanja kolone | 1mg |
Principi
- Utilizes silica column purification method.
- Uzorak se lizira i probavlja lizatom i proteazom, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nukleinska kiselina se adsorbira na membrani, while protein is not adsorbed and is removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Prednosti
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, itd.
- Brzo – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability – handle a variety of liquid samples
Sadržaj kompleta
| Sadržaj | D311302 | D311303 |
| Vremena pročišćavanja | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15ml cijevi za prikupljanje | 40 | 200 |
| Buffer ATL | 50 ml | 250 ml |
| Buffer AL | 50 ml | 250 ml |
| Međuspremnik GW1* | 22 ml | 110 ml |
| Međuspremnik GW2* | 12 ml | 50 ml |
| RNaza A | 20 mg | 90 mg |
| Proteinaza K | 100 mg | 440 mg |
| Pufer za otapanje proteaze | 10 ml | 30 ml |
| Međuspremnik AE | 20 ml | 120 ml |
Skladištenje i stabilnost
- Proteinase K and RNase A should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect their performance.
- Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) and remain stable for at least 18 mjeseci pod ovim uvjetima.
Experiment Data
Vodič za kupnju
| Ime | MAČKA BR | Količina uzorka | Leukocyte protocol* | Colum type | Elutio volume | Average yield | Vrijeme po vožnji |
| HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml stupca | ≥20μl | 5-9μg/200μl | ≤30 minuta |
| HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml stupca | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure Tissue&Krvni DNK Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml stupca | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 bunar ploča | 3-8μg/200μl |
Recenzije
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