Uvod
This product offers a fast and reliable method to isolate RNA, including miRNA, from a wide range of samples:
- Tissue
- Cells
- Blood
- Other clinical samples
The extracted RNA is suitable for various downstream applications, including:
- RT-PCR (Reverse Transcription PCR)
- Quantitative RT-PCR (qRT-PCR)
- Viral RNA detection
- Other RNA-based analyses
This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total RNA（miRNA）from tissue, and cell using two columns and DNase plus reagent |
| Prijave | RT-PCR, cDNA synthesis, second-generation sequencing |
| Proizvodi | RNA, miRNA |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Clinical tissues, cells, lymphocytes |
| Količina uzorka | Tissue: <20 mg Cells: <5 x 106 |
| Prinos | 2-50mg |
| Volumen eluacije | ≥30μl |
| Vrijeme po vožnji | ≤25 minutes |
Načelo
- Purification Method: Silica column purification is employed in this product for effective nucleic acid extraction.
- Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
- Sample Lysis and Digestion: The sample lysis process, coupled with proteinase K digestion, takes only 15 minuta, ensuring rapid processing.
- Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
- Nucleic Acid Adsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
- Elution: Finally, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.
Prednosti
- Efficient DNA removal – One-step RNA extraction can effectively remove genomic DNA
- High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Brzo – the whole extraction only takes 15-25 minuta
- Nontoxic – no toxic phenol-chloroform extraction is required in the extraction
Sadržaj kompleta
| Sadržaj | IVD4121 |
| Vremena pročišćavanja | 50 Pripreme |
| HiPure DNA Mini Column Ⅱ | 50 |
| HiPure RNA Mini Columns | 50 |
| 2ml cijevi za prikupljanje | 150 |
| Proteinaza K | 24 mg |
| Pufer za otapanje proteaze | 1.8 ml |
| DNase I | 600 μl |
| DNase Buffer | 6 ml |
| Buffer RTL | 40 ml |
| RNA Digestion Buffer | 15 ml |
| Buffer RWC* | 20 ml |
| Buffer RW2* | 20 ml |
| Nuclease Free Water | 10 ml |
Skladištenje i stabilnost
- Proteinase K Storage: Store Proteinase K at 2–8°C upon arrival. Short-term storage (do 8 tjedni) na sobnoj temperaturi (15–25°C) is acceptable without affecting its performance.
- DNase I Storage: Store DNase I at -20°C. Short-term storage (do 1 week) na sobnoj temperaturi (15–25°C) ne utječe na njegovu izvedbu.
- Remaining Kit Components Storage: Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15–25°C) and remain stable for at least 18 mjeseci pod ovim uvjetima.
Recenzije
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