Product Overview
- Suitable for rapid extraction of total DNA from various sources: tissue, cells, krv, saliva, swabs, blood spots, semen, and other clinical samples.
- Extracted DNA can be used directly for PCR, quantitative PCR, Southern Blot, virus DNA testing, and other applications.
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| Prijave | PCR, qPCR, Southern Bolt i otkrivanje virusa, itd |
| Metoda pročišćavanja | Mini spin column |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Tkivo, cells, krv, saliva, swabs, blood spots, semen, and other clinical samples |
| Količina uzorka | Solid tissue: 1-10mg; Anticoagulant blood: 200μl |
| Volumen eluacije | ≥20μl |
| Vrijeme po vožnji | 30 – 60 minuta |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 100µg |
Načelo
- Utilizes silica column purification method.
- Uzorak se lizira i probavlja lizatom i proteazom, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nukleinska kiselina se adsorbira na membrani, while protein is not adsorbed and removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Prednosti
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, itd.
- Brzo – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability- handle a variety of liquid samples
Sadržaj kompleta
| Sadržaj | D301802 | D301803 |
| Vremena pročišćavanja | 50 | 250 |
| HiPure DNA mini stupci I | 50 | 250 |
| 2ml cijevi za prikupljanje | 100 | 500 |
| Buffer ATL | 30 ml | 150 ml |
| Buffer AL | 30 ml | 150 ml |
| Buffer GW1 | 22 ml | 88 ml |
| Međuspremnik GW2 | 12 ml | 50 ml |
| Proteinaza K | 24 mg | 120 mg |
| Pufer za otapanje proteaze | 1.8 ml | 10 ml |
| Međuspremnik AE | 15 ml | 60 ml |
Skladištenje i stabilnost
- Proteinase K should initially be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect its performance.
- Preostale komponente kita mogu se čuvati na sobnoj temperaturi (15-25°C) and remain stable for at least 18 mjeseca.
- Alternatively, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
Experiment Data
Recenzije
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