MagPure A4 XP Features:
- Technology Base:
- Employs Magen’s solid-phase paramagnetic bead technology designed for the purification of PCR amplicons.
- High-Throughput Capability:
- Suitable for high-throughput settings, allowing for efficient simultaneous processing of multiple samples.
AmPure Technology:
- Selective Binding:
- Uses an optimized buffer that selectively binds PCR amplicons 100bp and larger to paramagnetic beads.
- Contaminant Removal:
- Simple washing procedure efficiently removes excess primers, nukleotidi, soli, and enzymes.
- Purity of PCR Product:
- Results in PCR products that are virtually free from contaminants, suitable for precise downstream applications.
pojedinosti
Tehnički podaci
Značajke | Tehnički podaci |
Glavne funkcije | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP) |
Prijave | NGS, DNA library |
Tehnologija pročišćavanja | Tehnologija magnetskih kuglica |
Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
Vrsta uzorka | DNA proizvodi, sustavi restrikcijskih endonukleaza, or other enzymatic reaction solutions |
Količina uzorka | Prikladno |
Oporavak | 80% |
Vrijeme rada | ≤50 minuta |
Načelo
Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- Contaminant Removal:
- Excess primers, nukleotidi, soli, and enzymes are removed through a simple washing procedure.
- DNA Elution:
- Purified DNA is eluted using Elution Buffer or Water.
Prednosti
- High recovery — up to 80%
- High throughput — using magnetic beads purification technology
Sadržaj kompleta
Sadržaj | BP-5 | BP-50 | BP-500 |
MagPure A4 XP | 5 ml | 50 ml | 500 ml |
Skladištenje i stabilnost
Storage and Handling Instructions:
- Storage Conditions:
- Store MagPure A4 XP at 2-8°C upon arrival.
- Stable for up to 18 mjeseci pod ovim uvjetima.
- Short-term Storage:
- Short-term storage (do 4 tjedni) na sobnoj temperaturi (15-25°C) does not affect performance.
- Reagent Preparation:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogeneous and consistent in color.
NEMOJTE ZAMRZAVATI.
Recenzije
Još nema recenzija.